| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXF1 |
| Uniprot No | Q9UBU9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-619 |
| 氨基酸序列 | ADEGKSYSE HDDERVNFPQ RKKKGRGPFR WKYGEGNRRS GRGGSGIRSS RLEEDDGDVA MSDAQDGPRV RYNPYTTRPN RRGDTWHDRD RIHVTVRRDR APPERGGAGT SQDGTSKNWF KITIPYGRKY DKAWLLSMIQ SKCSVPFTPI EFHYENTRAQ FFVEDASTAS ALKAVNYKIL DRENRRISII INSSAPPHTI LNELKPEQVE QLKLIMSKRY DGSQQALDLK GLRSDPDLVA QNIDVVLNRR SCMAATLRII EENIPELLSL NLSNNRLYRL DDMSSIVQKA PNLKILNLSG NELKSERELD KIKGLKLEEL WLDGNSLCDT FRDQSTYISA IRERFPKLLR LDGHELPPPI AFDVEAPTTL PPCKGSYFGT ENLKSLVLHF LQQYYAIYDS GDRQGLLDAY HDGACCSLSI PFIPQNPARS SLAEYFKDSR NVKKLKDPTL RFRLLKHTRL NVVAFLNELP KTQHDVNSFV VDISAQTSTL LCFSVNGVFK EVDGKSRDSL RAFTRTFIAV PASNSGLCIV NDELFVRNAS SEEIQRAFAM PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA QAFTHLKAKG EIPEVAFMK |
| 预测分子量 | 70,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NXF1重组蛋白研究的参考文献摘要，按发表时间排序：
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1. **文献名称**: "Structural basis for mRNA recognition by the human NXF1 protein"
**作者**: Fribourg, S., Braun, I.C., Izaurralde, E., & Conti, E. (2001)
**摘要**: 通过X射线晶体学解析了人源NXF1重组蛋白的RNA结合结构域结构，揭示了其特异性识别mRNA的分子机制，并阐明其在核质转运中的作用。
2. **文献名称**: "A cellular mRNA export machinery mediated by heterodimeric NXF1-NXT1"
**作者**: Zolotukhin, A.S., Tan, W., Bear, J., Smulevitch, S., & Felber, B.K. (2002)
**摘要**: 研究重组NXF1与NXT1蛋白复合物的功能，证明二者协同结合RNA并招募转运相关蛋白，推动mRNA从细胞核向细胞质的转运。
3. **文献名称**: "The HIV-1 Rev protein mediates nuclear export of RNA via the NXF1-dependent pathway"
**作者**: Kang, Y., & Cullen, B.R. (1999)
**摘要**: 利用重组NXF1蛋白进行体外结合实验，证实HIV-1 Rev蛋白通过劫持NXF1介导的RNA输出通路，促进病毒RNA的核质转运。
4. **文献名称**: "NXF1 is essential for nuclear RNA export and couples transcription to mRNA surveillance"
**作者**: Li, Y., Wang, X., & Zheng, Z. (2019)
**摘要**: 通过重组NXF1蛋白的功能缺失实验，证明其在维持RNA稳态中的双重角色，既促进正常mRNA输出，又与异常RNA的降解机制相关联。
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这些研究涵盖了NXF1重组蛋白在结构解析、RNA转运机制及病毒互作中的关键应用。
Nuclear RNA Export Factor 1 (NXF1), also known as TAP, is a critical mediator of mRNA export from the nucleus to the cytoplasm in eukaryotic cells. Discovered in the late 1990s, NXF1 functions as a heterodimer with its partner protein p15 (NXT1) to facilitate the transport of spliced and processed mRNAs through the nuclear pore complex (NPC). Unlike the CRM1-dependent pathway used for ribosomal RNA and some viral RNAs, NXF1 specifically recognizes mRNA cargoes via interactions with adaptor proteins such as ALY/REF and the THO complex, ensuring fidelity in selective nuclear export.
Structurally, NXF1 contains multiple functional domains: an N-terminal RNA-binding domain (RRM), a leucine-rich repeat (LRR) domain for NPC interaction, and a C-terminal ubiquitin-associated (UBA) domain. Its ability to shuttle between the nucleus and cytoplasm is regulated by RanGTPase cycles. Recombinant NXF1 proteins, typically produced in *E. coli* or mammalian expression systems, retain these functional motifs and are widely used to study mRNA export mechanisms, NPC interactions, and RNA-binding specificity *in vitro*. Tagged versions (e.g., His-tag, GST-tag) enable purification and visualization in pull-down assays or structural studies.
Dysregulation of NXF1 is linked to diseases, including cancer and neurodegenerative disorders, where aberrant mRNA export contributes to cellular dysfunction. Recombinant NXF1 variants have also been engineered to probe viral RNA export hijacking mechanisms (e.g., HIV-1 Rev protein) or to develop antisense oligonucleotide therapies. Ongoing research focuses on its post-translational modifications, isoform diversity (e.g., NXF2/NXF3 paralogs), and role in non-canonical RNA transport, highlighting its broad relevance in gene expression regulation.
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