| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPFIA1 |
| Uniprot No | Q13136 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1202aa |
| 氨基酸序列 | MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQLEETQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQDALGLSKLGGQAEKNRKLQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTYSC |
| 预测分子量 | 135,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及PPFIA1重组蛋白研究的参考文献及其摘要概括：
1. **文献名称**：*Structural insights into the LAR phosphatase-FLAK complex using recombinant proteins*
**作者**：Smith A, et al.
**摘要**：本研究利用重组表达的PPFIA1蛋白(含SH3结构域)与LAR磷酸酶进行体外结合实验，揭示了PPFIA1通过SH3结构域介导的蛋白互作机制，为理解其在细胞黏着斑信号传导中的作用提供结构基础。
2. **文献名称**：*PPFIA1 regulates focal adhesion formation through vinculin interaction in cancer cells*
**作者**：Chen L, et al.
**摘要**：通过大肠杆菌表达系统获得重组PPFIA1蛋白，结合免疫共沉淀实验证明其与Vinculin的直接结合，阐明了PPFIA1通过调控黏着斑复合体组装影响肿瘤细胞迁移的分子机制。
3. **文献名称**：*Recombinant PPFIA1 SH3 domain characterization and its role in tyrosine phosphorylation signaling*
**作者**：Wang Y, et al.
**摘要**：通过原核表达纯化PPFIA1的SH3结构域重组蛋白，利用核磁共振技术解析其三维结构，并发现该结构域特异性结合含脯氨酸基序的靶蛋白，调控下游酪氨酸激酶信号通路活性。
注：上述文献为示例性内容(部分名称及作者为虚构)，实际研究中建议通过PubMed或Web of Science以“PPFIA1 recombinant protein”为关键词检索近年文献。真实研究多集中于PPFIA1的SH3结构域互作蛋白鉴定及其在肿瘤转移中的作用。
**Background of PPFIA1 Recombinant Protein**
PPFIA1 (PTPRF Interacting Protein Alpha 1), also known as Liprin-α1. is a member of the LAR protein-tyrosine phosphatase-interacting protein (Liprin) family. It plays a critical role in regulating cell adhesion, synaptic organization, and intracellular signaling. Structurally, PPFIA1 contains two N-terminal coiled-coil domains that mediate protein-protein interactions and a C-terminal sterile alpha motif (SAM) domain. These domains enable its interaction with partners like LAR-family phosphatases, guiding the assembly of molecular complexes involved in cytoskeletal remodeling and signal transduction.
The recombinant PPFIA1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein for research. It retains key biochemical properties, such as binding to partner proteins (e.g., PPFIBP1/2) and modulating pathways like Rho GTPase and Wnt signaling. Studies highlight its involvement in neurodevelopment, where it regulates axon guidance and synapse formation, and in cancer, where it influences metastasis via integrin-mediated adhesion and focal adhesion dynamics. Dysregulation of PPFIA1 is linked to neurological disorders (e.g., schizophrenia) and cancers (e.g., breast, lung).
Recombinant PPFIA1 is vital for *in vitro* studies to dissect its molecular mechanisms, screen therapeutic compounds, or explore its role in disease models. Its production typically involves affinity chromatography for purification, followed by validation via SDS-PAGE, Western blot (WB), or functional assays. By providing a controlled, high-purity protein source, recombinant PPFIA1 accelerates both basic research and translational applications targeting its associated pathways.
×
