| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCDC1 |
| Uniprot No | P59894 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MAKTGAEDHR EALSQSSLSL LTEAMEVLQQ SSPEGTLDGN TVNPIYKYIL NDLPREFMSS QAKAVIKTTD DYLQSQFGPN RLVHSAAVSE GSGLQDCSTH QTASDHSHDE ISDLDSYKSN SKNNSCSISA SKRNRPVSAP VGQLRVAEFS SLKFQSARNW QKLSQRHKLQ PRVIKVTAYK NGSRTVFARV TVPTITLLLE ECTEKLNLNM AARRVFLADG KEALEPEDIP HEADVYVSTG EPFLNPFKKI KDHLLLIKKV TWTMNGLMLP TDIKRRKTKP VLSIRMKKLT ERTSVRILFF KNGMGQDGHE ITVGKETMKK VLDTCTIRMN LNLPARYFYD LYGRKIEDIS KGKH |
| 预测分子量 | 200,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCDC1重组蛋白的假设性参考文献示例(实际文献需通过学术数据库验证)：
1. **标题**: "DCDC1 Recombinant Protein Facilitates Ciliogenesis in Epithelial Cells"
**作者**: Smith A, et al.
**摘要**: 研究通过体外表达DCDC1重组蛋白，发现其通过调控微管结构促进纤毛发生，提示DCDC1在细胞极性维持中的作用。
2. **标题**: "Structural Characterization of DCDC1 and Its Role in Cancer Cell Motility"
**作者**: Lee J, et al.
**摘要**: 利用重组DCDC1蛋白解析其晶体结构，发现其与细胞骨架蛋白互作，可能通过增强迁移能力促进肿瘤转移。
3. **标题**: "DCDC1 Recombinant Expression in Prokaryotic Systems: Challenges and Optimization"
**作者**: Zhang Y, et al.
**摘要**: 报道了DCDC1重组蛋白在大肠杆菌中的可溶性表达策略，通过密码子优化和纯化条件改进获得高活性蛋白。
4. **标题**: "DCDC1 Knockdown and Recombinant Rescue in Neuronal Development Models"
**作者**: Gupta R, et al.
**摘要**: 通过重组DCDC1蛋白回补实验，验证其在神经元突触形成中的必要性，为神经发育疾病机制提供依据。
**注意**：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词"DCDC1 recombinant protein"或联系领域数据库获取。若研究较新，建议补充预印本平台(如bioRxiv)或专利数据库信息。
DCDC1 (Doublecortin Domain-Containing Protein 1) is a microtubule-associated protein implicated in cellular processes such as ciliogenesis, cell migration, and cytoskeletal organization. It belongs to the doublecortin family, characterized by conserved doublecortin (DCX) domains that facilitate interactions with microtubules. DCDC1 is predominantly expressed in ciliated cells and plays a critical role in the formation and function of primary cilia, sensory organelles essential for signal transduction in development and tissue homeostasis. Dysregulation of DCDC1 has been linked to ciliopathies, developmental disorders, and certain cancers, highlighting its importance in cellular and physiological contexts.
Recombinant DCDC1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in bacterial or mammalian expression systems, the protein retains functional domains, including its DCX motifs, to enable interaction with microtubules. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize this protein to investigate its binding partners, structural dynamics, and enzymatic activities, such as microtubule stabilization or polymerization modulation. It also serves as a tool for studying cilia-related pathways, including Wnt and Hedgehog signaling, and for screening potential therapeutic agents targeting ciliopathies or cancer.
Current research on recombinant DCDC1 focuses on resolving its 3D structure, post-translational modifications, and disease-associated mutations. Its applications extend to cell-based assays, structural biology, and disease modeling, offering insights into both basic biology and translational medicine. By enabling precise manipulation of DCDC1 in controlled settings, recombinant protein technology continues to advance our understanding of its role in health and disease.
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