| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF26 |
| Uniprot No | P17031 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 335-533aa |
| 氨基酸序列 | MHTGEKPYQCSDCGKAFNMKTQLIVHQGVHTGNNPYQCGECGKAFGRKEQLTAHLRAHAGEKPYGCSECGKAFSSKSYLVIHRRTHTGERPYECSLCERAFCGKSQLIIHQRTHSTEKPYECNECEKAYPRKASLQIHQKTHSGEKPFKCSECGKAFTQKSSLSEHQRVHTGEKPWKCSECGKSFCWNSGLRIHRKTHK |
| 预测分子量 | 38.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF26重组蛋白的参考文献示例(注：部分内容为模拟概括，实际文献可能需要通过学术数据库核实)：
1. **《Cloning and Expression of Human ZNF26 in Escherichia coli》**
- **作者**: Smith A, et al.
- **摘要**: 本研究报道了ZNF26基因的克隆及其在大肠杆菌中的重组表达。通过优化表达条件，成功纯化出具有DNA结合活性的重组ZNF26蛋白，并验证其与特定基因启动子区域的相互作用。
2. **《Structural Analysis of Zinc Finger Protein ZNF26 Using X-ray Crystallography》**
- **作者**: Lee B, et al.
- **摘要**: 利用重组ZNF26蛋白进行晶体结构解析，揭示了其锌指结构域的精确三维构象，为理解其与DNA结合的功能机制提供了结构基础。
3. **《ZNF26 Recombinant Protein Suppresses Tumor Cell Proliferation in vitro》**
- **作者**: Chen C, et al.
- **摘要**: 通过体外实验发现，重组ZNF26蛋白能够抑制多种癌细胞的增殖，可能与调控细胞周期相关基因的转录抑制有关。
4. **《Functional Characterization of ZNF26 as a Transcriptional Repressor》**
- **作者**: García-Ruiz S, et al.
- **摘要**: 研究利用重组ZNF26蛋白进行染色质免疫沉淀(ChIP)分析，证实其在基因调控中作为转录抑制因子，靶向特定DNA序列并招募组蛋白去乙酰化酶(HDACs)。
**提示**：以上文献为示例性质，建议通过PubMed或Web of Science以“ZNF26 recombinant protein”为关键词检索最新研究。若文献有限，可扩展至锌指蛋白家族(如ZNFs)的重组表达相关研究。
ZNF26. a member of the zinc finger protein (ZFP) family, is a transcription factor characterized by its Kruppel-associated box (KRAB) domain and multiple C2H2-type zinc finger motifs. As part of the KRAB-ZFP subfamily, it plays roles in transcriptional regulation, potentially repressing gene expression by recruiting chromatin-modifying complexes through its KRAB domain. The protein is encoded by the ZNF26 gene located on human chromosome 12 (12q24.31) and is evolutionarily conserved, suggesting functional significance in cellular processes.
Recombinant ZNF26 protein is engineered using expression systems (e.g., E. coli, mammalian, or insect cells) to enable functional studies. Its production typically involves cloning the coding sequence into expression vectors, followed by purification via affinity tags (e.g., His-tag). This recombinant approach allows researchers to investigate ZNF26's DNA-binding specificity, protein-protein interactions, and regulatory mechanisms in vitro. Structural studies using recombinant protein have helped map its zinc finger domains, which likely recognize specific DNA sequences, and its nuclear localization signals.
Biologically, ZNF26 has been implicated in cancer progression and cellular differentiation, though its exact targets remain partially characterized. Epigenetic studies suggest it may silence retrotransposons or regulate developmental genes. The recombinant form enables high-throughput screening for small molecules modulating its activity, with potential therapeutic applications in oncology and genetic disorders. Current research focuses on clarifying its physiological roles and validating its utility as a biomarker or drug target.
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