| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF581 |
| Uniprot No | Q9P0T4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MASMTGGQQMGRGEFMLVLPSPCPQPLAFSSVETMEGPPRRTCRSPEPGP SSSIGSPQASSPPR PNHYLLIDTQGVPYTVLVDEESQREPGASGAPGQ KKCYSCPVCSRVFEYMSYLQRHSITHSEVK PFECDICGKAFKRASHLA RHHSIHLAGGGRPHGCPLCPRRFRDAGELAQHSRVHSGERPFQCPH CP RRFMEQNTLQKHTRWKHP |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF581重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **标题**: "ZNF581 Recombinant Protein Suppresses Tumor Growth via Epigenetic Regulation"
**作者**: L. Chen et al.
**摘要**: 本研究揭示了ZNF581重组蛋白通过结合特定DNA启动子区域并招募组蛋白去乙酰化酶(HDACs)，抑制肿瘤相关基因表达，从而在体外和小鼠模型中显著抑制结直肠癌细胞增殖。
2. **标题**: "Structural Characterization of ZNF581 and Its Interaction with p53"
**作者**: M. Tanaka & S. Kumar
**摘要**: 通过X射线晶体学解析ZNF581重组蛋白的锌指结构域，发现其通过C末端与p53蛋白直接互作，增强p53的稳定性，提示其在DNA损伤修复通路中的潜在调控作用。
3. **标题**: "ZNF581 Recombinant Protein Attenuates Autoimmune Inflammation by Modulating T-cell Differentiation"
**作者**: E. Rodriguez et al.
**摘要**: 实验表明，外源性ZNF581蛋白可抑制Th17细胞分化并促进Treg细胞生成，显著降低小鼠类风湿性关节炎模型的炎症评分，提示其作为免疫调节剂的治疗潜力。
4. **标题**: "High-yield Production of Functional ZNF581 in E. coli: Purification and Functional Assay Optimization"
**作者**: K. Park & J. Lee
**摘要**: 开发了一种新型大肠杆菌表达系统，通过密码子优化和温度调控实现ZNF581重组蛋白的高效可溶性表达，并经凝胶迁移实验(EMSA)验证其体外DNA结合活性。
注：以上内容为学术模拟，实际文献需通过PubMed/Web of Science等平台检索确认。
ZNF581 recombinant protein is derived from the human ZNF581 gene, which encodes a zinc finger protein belonging to the Krüppel-associated box (KRAB) domain-containing family. Zinc finger proteins (ZFPs) are characterized by tandem repeats of cysteine and histidine residues that form structural motifs capable of binding DNA, RNA, or other proteins. ZNF581. specifically, contains multiple C2H2-type zinc finger domains and a KRAB domain, which is typically involved in transcriptional repression by recruiting chromatin-modifying complexes. This protein is thought to play regulatory roles in gene expression, particularly in processes like cell differentiation, proliferation, and apoptosis.
Recombinant ZNF581 is produced using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The purified protein retains functional domains critical for DNA binding and protein-protein interactions, making it a valuable tool for studying its biological mechanisms. Researchers utilize it in assays such as electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), or in vitro binding studies to map interaction partners and target genes.
Emerging evidence links ZNF581 to cancer biology, where it may act as a tumor suppressor or oncogene depending on cellular context. Dysregulation of ZNF581 has been observed in malignancies like hepatocellular carcinoma and colorectal cancer, suggesting involvement in pathways like Wnt/β-catenin or p53 signaling. Its recombinant form enables exploration of these roles, including epigenetic regulation via KRAB-mediated recruitment of histone deacetylases (HDACs) or other modifiers.
Overall, ZNF581 recombinant protein serves as a key reagent for dissecting the molecular functions of zinc finger proteins in development and disease, with potential applications in drug discovery and biomarker development.
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