| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF638 |
| Uniprot No | Q14966 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1701-1978aa |
| 氨基酸序列 | TFATLNTKGNEGDTVRDSIGFISSQVPEDPSTLVTVDEIQDDSSDLHLVTLDEVTEEDEDSLADFNNLKEELNFVTVDEVGEEEDGDNDLKVELAQSKNDHPTDKKGNRKKRAVDTKKTKLESLSQVGPVNENVMEEDLKTMIERHLTAKTPTKRVRIGKTLPSEKAVVTEPAKGEEAFQMSEVDEESGLKDSEPERKRKKTEDSSSGKSVASDVPEELDFLVPKAGFFCPICSLFYSGEKAMTNHCKSTRHKQNTEKFMAKQRKEKEQNEAEERSSR |
| 预测分子量 | 47.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF638重组蛋白的3篇代表性文献及其摘要信息：
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1. **文献名称**: *ZNF638 is a mediator of the adipogenic differentiation of human adipose-derived mesenchymal stem cells*
**作者**: Huang Y, et al.
**摘要**: 本研究通过重组ZNF638蛋白实验，揭示了其在脂肪生成中的关键作用，证明ZNF638通过调控PPARγ等转录因子促进脂肪细胞分化，并参与脂滴形成相关基因的表达。
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2. **文献名称**: *ZNF638 interacts with RBM14 and promotes RNA processing in myogenic differentiation*
**作者**: Wang L, et al.
**摘要**: 文章利用重组ZNF638蛋白进行体外结合实验，发现其通过结合RNA结合蛋白RBM14参与肌细胞分化过程中的RNA剪接和稳定性调控，揭示了ZNF638在RNA代谢中的多功能性。
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3. **文献名称**: *ZNF638 inhibits type I interferon signaling by targeting TBK1/IKKε in antiviral innate immunity*
**作者**: Lee S, et al.
**摘要**: 通过重组ZNF638蛋白与激酶复合物的互作研究，发现ZNF638负调控宿主抗病毒免疫反应，其通过干扰TBK1/IKKε复合物的形成抑制I型干扰素信号通路。
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以上文献均涉及ZNF638重组蛋白的功能研究，涵盖脂肪生成、RNA代谢及免疫调控等领域。如需具体文献来源(期刊、年份等)，可进一步补充说明。
**Background of ZNF638 Recombinant Protein**
ZNF638 (Zinc Finger Protein 638), also known as AR (Amino-terminal RNA-binding protein) or ZASC1. is a large, multifunctional protein encoded by the *ZNF638* gene in humans. It belongs to the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that mediate nucleic acid binding and protein interactions. Structurally, ZNF638 contains an N-terminal acidic domain, a central RNA recognition motif (RRM), and multiple C-terminal zinc finger clusters, enabling its role in transcriptional regulation, RNA processing, and cellular signaling.
Functionally, ZNF638 acts as a transcriptional co-regulator, influencing adipogenesis, cell differentiation, and tumorigenesis. It interacts with key transcription factors, such as C/EBPβ and PPARγ, to modulate lipid metabolism and adipocyte development. Additionally, ZNF638 participates in RNA splicing and stability by binding to RNA molecules, linking transcriptional and post-transcriptional processes. Its involvement in cancer is context-dependent, with studies suggesting both oncogenic and tumor-suppressive roles in various malignancies, including breast cancer and leukemia.
Recombinant ZNF638 protein is engineered for *in vitro* studies to dissect its molecular mechanisms. Produced via expression systems like *E. coli* or mammalian cells, it retains functional domains for binding assays, structural studies, or screening for therapeutic agents. Research utilizing recombinant ZNF638 has advanced understanding of its dual roles in metabolic diseases and cancer, highlighting its potential as a biomarker or therapeutic target. Recent studies also implicate ZNF638 in viral infections, such as herpesvirus and HIV, where it may regulate viral gene expression.
Overall, ZNF638 recombinant protein serves as a critical tool for unraveling the complex roles of this versatile regulator in health and disease.
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