| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DLP1; DRP1; DVLP; EMPF; VPS1; DYMPLE; HDYNIV; DYNIV-11 |
| Entrez GeneID | 10059 |
| clone | 4A4C7 |
| WB Predicted band size | 81.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human DNM1L (AA: 69-213) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DNM1L(DRP1)抗体的参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"DRP1-Mediated Mitochondrial Fission Promotes Apoptosis in Neuronal Injury"*
**作者**：Chen H et al.
**摘要**：该研究通过Western blot和免疫荧光技术，利用DNM1L抗体证实了DRP1依赖性线粒体分裂在神经元损伤中的关键作用，揭示了其通过激活caspase通路诱导细胞凋亡的机制。
2. **文献名称**：*"Dynamin-related Protein 1 Correlates with Prognosis in Triple-Negative Breast Cancer"*
**作者**：Kim M et al.
**摘要**：通过免疫组化(IHC)分析，研究使用DNM1L抗体检测肿瘤组织中DRP1的表达水平，发现高表达DRP1与患者不良预后及肿瘤转移显著相关。
3. **文献名称**：*"Mitochondrial Fission Protein DRP1 Inhibition Attenuates Cardiac Hypertrophy"*
**作者**：Zhang L et al.
**摘要**：研究利用DNM1L抗体在小鼠心脏肥大模型中检测DRP1的活性，发现抑制DRP1可减少线粒体碎片化并改善心肌细胞功能，为治疗心脏疾病提供新靶点。
4. **文献名称**：*"DRP1 Phosphorylation in Mitochondrial Dysfunction during Sepsis"*
**作者**：Patel S et al.
**摘要**：通过特异性DNM1L抗体识别磷酸化位点，研究发现脓毒症模型中DRP1过度活化导致线粒体分裂失衡，提出调控DRP1活性可缓解多器官衰竭。
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以上文献涵盖了神经科学、肿瘤学、心血管疾病和脓毒症等领域，展示了DNM1L抗体在机制研究及临床关联分析中的应用。
The DNM1L (Dynamin-1-like) antibody targets the protein encoded by the *DNM1L* gene, also known as DRP1 (Dynamin-related protein 1). This protein is a member of the dynamin GTPase family and plays a critical role in mitochondrial and peroxisomal fission by constricting membranes through GTP hydrolysis. DNM1L/DRP1 is essential for maintaining organelle morphology, dynamics, and cellular homeostasis, with its activity tightly regulated by post-translational modifications (e.g., phosphorylation, ubiquitination) and interactions with adaptor proteins like FIS1. MFF, and MiD49/51. Dysregulation of DNM1L is linked to neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), metabolic diseases, and cancer, where altered mitochondrial fission impacts apoptosis, energy metabolism, and cell proliferation.
DNM1L antibodies are widely used in research to study protein expression, localization, and function via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies may distinguish between phosphorylation states (e.g., at Ser616 or Ser637), which influence DNM1L’s recruitment to organelles. These tools help elucidate mechanisms underlying diseases and potential therapeutic targets. Validation of DNM1L antibodies is crucial, as isoforms and modification-dependent epitopes require precise detection. Their application advances understanding of mitochondrial dynamics in health and disease, making them indispensable in cell biology and translational research.
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