| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMG14 |
| Uniprot No | P05114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MPKRKVSSTEGAAKEEPKRRSARLSAKPPAKVEAKPKKAAAKDKSSDKKV QTKGKRGAKGKQAEVANQETKEDLPAENGETKTEESPASDEAGEKEAKSD |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMG14重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant HMG14 proteins: DNA-binding and nucleosome-binding activities"**
- **作者**: Postnikov, Y.V., et al.
- **摘要**: 该研究报道了通过大肠杆菌表达系统制备重组HMG14蛋白的方法，并分析了其与DNA及核小体的结合特性。实验表明HMG14通过特异性相互作用改变染色质结构，增强转录因子的可及性。
2. **"Interaction of HMG14 with mononucleosomes and nucleosome core particles"**
- **作者**: Trieschmann, L., et al.
- **摘要**: 通过重组HMG14蛋白研究其与核小体的相互作用，发现HMG14优先结合于核小体核心颗粒的连接DNA区域，并可能通过竞争组蛋白H1的结合位点调节染色质压缩状态。
3. **"Phosphorylation of recombinant HMG14 by protein kinase CK2 modulates its DNA-binding properties"**
- **作者**: Kuehlers, V., et al.
- **摘要**: 研究重组HMG14的翻译后修饰对其功能的影响，发现CK2激酶介导的磷酸化修饰显著降低HMG14与线性DNA的结合能力，但增强其在染色质重塑复合体中的协同作用。
以上文献涵盖HMG14重组蛋白的制备、功能机制及其修饰调控，聚焦于其在染色质动态调节中的作用。
**Background of Recombinant HMG14 Protein**
High Mobility Group (HMG) proteins are a family of non-histone chromosomal proteins involved in DNA organization, transcription regulation, and chromatin remodeling. Among them, the HMG-14/17 subfamily (also termed HMG-N) specifically binds to nucleosomes, influencing chromatin structure and facilitating transcriptional activation. HMG14. encoded by the *HMGN1* gene in humans, is a small (~10 kDa), highly conserved nuclear protein characterized by a nucleosome-binding domain and a chromatin-unfolding motif.
Recombinant HMG14 refers to the protein produced via genetic engineering techniques, often expressed in bacterial systems (e.g., *E. coli*) for research applications. Its recombinant form retains the native ability to interact with nucleosomal DNA, modulating chromatin accessibility for transcription factors or repair machinery. Studies highlight its role in epigenetic regulation, as HMG14 binding is associated with acetylated histones (e.g., H3K14ac), a hallmark of transcriptionally active chromatin.
Research on recombinant HMG14 has provided insights into DNA damage response, gene expression, and cellular differentiation. It serves as a tool to study chromatin dynamics in vitro, such as nucleosome remodeling assays. Dysregulation of HMG14 has been implicated in diseases like cancer and neurodegenerative disorders, linking chromatin structure anomalies to pathological states.
In summary, recombinant HMG14 is a vital reagent for probing chromatin-related mechanisms, bridging structural biology with functional genomics. Its applications span basic research, drug discovery, and therapeutic targeting of chromatin-associated diseases.
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