| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDGFL1 |
| Uniprot No | Q5TGJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-251aa |
| 氨基酸序列 | MSAYGMPMYK SGDLVFAKLK GYAHWPARIE HMTQPNRYQV FFFGTHETAF LSPKRLFPYK ECKEKFGKPN KRRGFSAGLW EIENNPTVQA SDCPLASEKG SGDGPWPEPE AAEGDEDKPT HAGGGGDELG KPDDDKPTEE EKGPLKRSAG DPPEDAPKRP KEAAPDQEEE AEAERAAEAE RAAAAAAATA VDEESPFLVA VENGSAPSEP GLVCEPPQPE EEELREEEVA DEEASQEWHA EAPGGGDRDS L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDGFL1重组蛋白的参考文献示例(注：以下内容为虚拟示例，实际文献需通过学术数据库查询)：
1. **文献名称**：Expression and Functional Analysis of Recombinant HDGFL1 in Cancer Cells
**作者**：Lee, J. et al. (2018)
**摘要**：该研究成功在哺乳动物细胞中表达了重组HDGFL1蛋白，并发现其通过激活MAPK信号通路促进肿瘤细胞的增殖和迁移，提示其在癌症进展中的潜在作用。
2. **文献名称**：Structural Characterization of HDGFL1 Recombinant Protein Derived from E. coli
**作者**：Zhang, Y. et al. (2020)
**摘要**：作者利用大肠杆菌系统高效表达并纯化了HDGFL1重组蛋白，通过X射线晶体学解析其三维结构，揭示了其N端结构域在DNA结合中的关键功能。
3. **文献名称**：HDGFL1 Recombinant Protein Modulates Neuronal Differentiation in vitro
**作者**：Smith, R.K. et al. (2019)
**摘要**：研究发现，外源性添加HDGFL1重组蛋白可增强神经干细胞的分化能力，表明其在神经发育和再生医学中的潜在应用价值。
4. **文献名称**：Interaction between HDGFL1 and Chromatin Remodeling Complexes Revealed by Pull-down Assays
**作者**：Wang, H. et al. (2021)
**摘要**：通过重组HDGFL1蛋白的Pull-down实验，鉴定出与多个染色质重塑复合体的相互作用，提示其可能通过表观遗传调控影响基因表达。
(注：实际文献需根据具体研究内容检索，建议使用PubMed、Web of Science等平台以“HDGFL1 recombinant protein”为关键词查询。)
**Background of HDGFL1 Recombinant Protein**
HDGFL1 (Hepatoma-Derived Growth Factor-Like 1) is a member of the HDGF family of proteins, which are characterized by a conserved N-terminal HATH (Hepatoma-Derived Growth Factor/HDGF-Related Proteins) domain involved in DNA binding and protein interactions. Unlike its well-studied relative HDGF, HDGFL1 remains less characterized but shares structural homology, suggesting roles in cell proliferation, differentiation, and tissue development.
HDGFL1 is expressed in various tissues, including the brain, liver, and reproductive organs, and is implicated in embryonic development and tissue repair. Studies suggest it may regulate signaling pathways linked to Wnt/β-catenin or MAPK, influencing cellular responses to stress or injury. Its interaction with nucleic acids and other proteins hints at potential involvement in transcriptional regulation or chromatin remodeling.
Recombinant HDGFL1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling functional studies. Purification often involves affinity tags (e.g., His-tag) for high yield and purity. Researchers utilize this protein to investigate its biochemical properties, binding partners, and mechanisms in physiological and pathological contexts.
Emerging evidence links HDGFL1 dysregulation to diseases such as cancer, neurodevelopmental disorders, and fibrosis, highlighting its therapeutic or diagnostic potential. For example, elevated HDGFL1 in certain tumors may promote cell survival or metastasis. Recombinant HDGFL1 also serves as an antigen for antibody development or a standard in biomarker assays.
Overall, HDGFL1 recombinant protein is a critical tool for unraveling the biological significance of this understudied protein, bridging gaps in understanding its role in health and disease.
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