| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDGFRP3 |
| Uniprot No | Q9Y3E1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-203aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSH MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFG THETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQ AIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSY TSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTS EGT |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDGFRP3重组蛋白的3篇文献示例(注：部分内容为模拟概括，供参考)：
1. **《Expression and Functional Analysis of HDGFRP3 in Hepatocellular Carcinoma》**
- 作者：Li, X. et al.
- 摘要：研究利用大肠杆菌系统成功表达并纯化HDGFRP3重组蛋白，发现其通过激活PI3K/AKT信号通路促进肝癌细胞增殖和迁移，为肝癌治疗提供潜在靶点。
2. **《Structural Characterization of HDGFRP3 Recombinant Protein and Its Role in Neuronal Development》**
- 作者：Smith, J.R. & Patel, K.
- 摘要：通过X射线晶体学解析HDGFRP3重组蛋白结构，揭示其N端结构域对神经元突触形成的调控作用，并验证其在体外促进神经细胞分化的功能。
3. **《HDGFRP3 Recombinant Protein Enhances DNA Repair Efficiency in Lung Cancer Cells》**
- 作者：Wang, Y. et al.
- 摘要：利用哺乳动物表达系统制备HDGFRP3重组蛋白，发现其通过与DNA损伤修复蛋白Ku80相互作用，提高肺癌细胞对放疗的耐受性，提示其临床预后价值。
4. **《Development of a High-Yield HDGFRP3 Recombinant Protein Production System Using Pichia pastoris》**
- 作者：Gomez, A.L. et al.
- 摘要：优化毕赤酵母表达系统实现HDGFRP3重组蛋白的高效分泌表达，验证其生物活性并建立规模化纯化工艺，为后续药物筛选奠定基础。
(注：以上文献为模拟概括，实际研究中请通过PubMed或Web of Science等平台检索真实文献。)
**Background of HDGFRP3 Recombinant Protein**
Hepatoma-derived growth factor-related protein 3 (HDGFRP3), also known as HDGF-related protein 3. belongs to the HDGF family of proteins characterized by a conserved N-terminal PWWP domain and a C-terminal nuclear localization signal. This family plays roles in cell proliferation, differentiation, and tissue development by modulating gene expression and chromatin interactions. HDGFRP3. specifically, is implicated in regulating transcriptional activity and chromatin remodeling, though its precise molecular mechanisms remain less defined compared to other members like HDGF or HRP2.
Recombinant HDGFRP3 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian expression systems) to ensure high purity and biological activity. Its production often involves tagging (e.g., His-tag) for efficient purification. Structurally, the recombinant protein retains functional domains critical for DNA or protein interactions, enabling studies on its role in cellular processes.
Research highlights HDGFRP3’s involvement in cancer progression, particularly in tumors like gliomas and hepatocellular carcinoma, where it may promote cell survival and invasiveness. Additionally, it interacts with chromatin-modifying complexes, suggesting a role in epigenetic regulation. Recombinant HDGFRP3 serves as a tool to investigate these pathways, screen therapeutic agents, or develop diagnostic biomarkers.
Despite its potential, HDGFRP3’s functions are understudied, necessitating further exploration into its binding partners, signaling networks, and therapeutic relevance. The availability of recombinant HDGFRP3 accelerates mechanistic studies and translational applications in oncology and regenerative medicine.
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