| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ERF1; TFAP2G; hAP-2g; AP2-GAMMA |
| Entrez GeneID | 7022 |
| clone | 2F12A6 |
| WB Predicted band size | 49.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TFAP2C (AA: 341-450) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与TFAP2C抗体相关的参考文献概览：
1. **文献名称**：*TFAP2C regulates transcription in human keratinocytes and mammary epithelial cells*
**作者**：Woodfield et al.
**摘要**：该研究利用TFAP2C抗体通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了TFAP2C在表皮细胞和乳腺癌细胞中的转录调控作用，发现其通过结合特定基因启动子调控细胞增殖和分化。
2. **文献名称**：*AP-2γ is required for maintenance of pluripotency in embryonic stem cells*
**作者**：Rahl et al.
**摘要**：通过免疫荧光和流式细胞术(使用TFAP2C抗体)，文章证明TFAP2C在小鼠胚胎干细胞多能性维持中起关键作用，其缺失导致分化相关基因异常激活。
3. **文献名称**：*TFAP2C expression in human tumors: A tissue microarray study*
**作者**：Pellikainen et al.
**摘要**：该研究使用TFAP2C抗体对多种肿瘤组织进行免疫组化分析，发现其在乳腺癌、卵巢癌和睾丸生殖细胞肿瘤中高表达，提示其作为潜在肿瘤标志物的价值。
这些文献均通过TFAP2C抗体探究该蛋白在疾病或发育中的功能，涵盖实验技术如ChIP、Western blot及临床样本分析。如需具体文献链接或补充，可进一步提供研究方向细化检索。
TFAP2C, also known as transcription factor AP-2 gamma (TFAP2γ), is a member of the AP-2 family of transcription factors, which play critical roles in embryonic development, cell differentiation, and tissue-specific gene regulation. TFAP2C contains a characteristic helix-span-helix dimerization domain and a basic DNA-binding region, enabling it to bind specific DNA sequences (e.g., GC-rich motifs) and regulate target gene expression. It is essential for placental development, kidney morphogenesis, and epidermal differentiation. Dysregulation of TFAP2C has been linked to cancers, including breast, ovarian, and lung cancers, where it often acts as an oncogene by promoting cell proliferation, survival, and metastasis.
Antibodies against TFAP2C are widely used in research to study its expression patterns, molecular interactions, and functional roles. These antibodies are typically validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Commercial TFAP2C antibodies are often raised against epitopes within its N-terminal or C-terminal regions. Specificity is confirmed using knockout cell lines or siRNA-mediated knockdown to ensure minimal cross-reactivity with other AP-2 family members (e.g., TFAP2A or TFAP2B). Researchers utilize these tools to explore TFAP2C’s involvement in developmental pathways, cancer biology, and stem cell maintenance, as well as its interplay with signaling cascades like Wnt/β-catenin and hormonal pathways. Reliable TFAP2C antibodies are critical for advancing both basic research and clinical studies targeting TFAP2C-associated diseases.
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