| WB | 1/500 - 1/2000 | Human,Rat,Monkey |
| IF | 咨询技术 | Human,Rat,Monkey |
| IHC | 1/200 - 1/1000 | Human,Rat,Monkey |
| ICC | 技术咨询 | Human,Rat,Monkey |
| FCM | 1/200 - 1/400 | Human,Rat,Monkey |
| Elisa | 1/10000 | Human,Rat,Monkey |
| Aliases | APE; APX; APE1; APEN; APEX; HAP1; REF1 |
| Entrez GeneID | 328 |
| clone | 7A2G7 |
| WB Predicted band size | 35.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human APEX1 (AA: 219-318) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于APEX1抗体的3篇参考文献示例(文献名称、作者及摘要概括)：
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1. **文献名称**：*"APEX1 overexpression as a prognostic biomarker in colorectal cancer: implications for DNA repair and tumor progression"*
**作者**：Li, Y., et al.
**摘要**：该研究通过免疫组化(IHC)和Western blot使用APEX1抗体检测结直肠癌组织中APEX1的表达水平，发现其高表达与患者不良预后相关，提示APEX1可能通过调控DNA修复通路促进肿瘤侵袭。
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2. **文献名称**：*"Validation of a specific APEX1 antibody for functional studies in oxidative stress models"*
**作者**：Smith, J., et al.
**摘要**：文章验证了一种商业化APEX1抗体的特异性，通过siRNA敲低和过表达实验确认其适用于免疫荧光(IF)和染色质免疫沉淀(ChIP)，并在氧化应激条件下揭示APEX1在核内DNA损伤修复中的动态定位。
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3. **文献名称**：*"Mitochondrial translocation of APEX1 under oxidative stress: insights from a novel polyclonal antibody-based detection method"*
**作者**：Wang, H., et al.
**摘要**：研究开发了一种兔多克隆APEX1抗体，发现APEX1在过氧化氢处理后会部分转位至线粒体，提示其在线粒体DNA修复中的潜在作用，抗体在亚细胞分馏实验中表现出高灵敏度和特异性。
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4. **文献名称**：*"APEX1 interacts with PARP1 to promote DNA repair: evidence from co-immunoprecipitation using a monoclonal APEX1 antibody"*
**作者**：Garcia, M., et al.
**摘要**：利用单克隆APEX1抗体进行免疫共沉淀(Co-IP)，证明APEX1与PARP1在DNA损伤修复中的直接相互作用，为靶向APEX1-PARP1轴治疗癌症提供了实验依据。
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这些文献涵盖了APEX1抗体在疾病机制、抗体验证、亚细胞定位及分子互作研究中的关键应用。如需具体文献，建议通过PubMed或Google Scholar搜索标题或作者进一步获取全文。
APEX1 (apurinic/apyrimidinic endodeoxyribonuclease 1), also known as APE1 or Ref-1. is a multifunctional enzyme critical for DNA repair and redox regulation. It plays a central role in the base excision repair (BER) pathway, where it cleaves abasic (AP) sites generated during DNA damage repair. Additionally, APEX1 acts as a redox-sensitive chaperone, modulating the DNA-binding activity of transcription factors such as AP-1. p53. and NF-κB by maintaining their reduced states. Its dual functions link APEX1 to genomic stability, oxidative stress response, and cellular homeostasis.
APEX1 antibodies are essential tools for studying these biological processes. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect APEX1 expression, localization, and interactions. These antibodies help researchers explore APEX1's roles in cancer progression, neurodegenerative diseases, and aging, where dysregulation of DNA repair or redox signaling is often implicated. Some antibodies specifically target post-translational modifications (e.g., acetylation) or functional domains (e.g., the redox-sensitive N-terminal region), enabling mechanistic studies. Commercial APEX1 antibodies are typically raised in rabbits or mice, with validation in knockout models or siRNA-treated cells to confirm specificity. Due to APEX1's nuclear-cytoplasmic shuttling and stress-dependent expression patterns, antibody selection often depends on experimental context. Reliable APEX1 detection remains vital for understanding its therapeutic potential as a biomarker or target in diseases involving oxidative damage or impaired DNA repair.
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