| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Havcr1 |
| Uniprot No | Q96D42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-290aa |
| 氨基酸序列 | SVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPMTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTA |
| 预测分子量 | 59.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HAVCR1(TIM-1)重组蛋白的相关文献摘要：
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1. **文献名称**: *"TIM-1 is a ligand of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and co-stimulates T cell activation"*
**作者**: **Liu Y et al.**
**摘要**: 本研究利用重组HAVCR1/TIM-1蛋白，通过体外结合实验和T细胞共培养模型，揭示了TIM-1与CEACAM1的相互作用机制，证明其可通过协同刺激信号增强T细胞活化和炎症反应，为自身免疫疾病治疗提供潜在靶点。
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2. **文献名称**: *"Recombinant TIM-1 extracellular domain modulates allergic lung inflammation by binding to phosphatidylserine on apoptotic cells"*
**作者**: **Meyers JH et al.**
**摘要**: 作者通过表达重组TIM-1胞外域蛋白，发现其能够特异性识别凋亡细胞表面的磷脂酰丝氨酸(PS)，并抑制哮喘模型中的Th2型免疫应答，提示TIM-1重组蛋白在调控过敏性炎症中的潜在治疗价值。
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3. **文献名称**: *"Structural basis of TIM-1-mediated hepatitis A virus entry"*
**作者**: **Wang X et al.**
**摘要**: 通过重组表达HAVCR1的IgV结构域蛋白并结合冷冻电镜技术，解析了其与甲型肝炎病毒(HAV)衣壳蛋白的复合物结构，阐明了病毒入侵宿主细胞的分子机制，为抗病毒药物设计提供了结构基础。
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4. **文献名称**: *"Recombinant TIM-1-Fc fusion protein attenuates renal ischemia-reperfusion injury by inhibiting T cell proliferation"*
**作者**: **Zhang L et al.**
**摘要**: 研究构建了TIM-1胞外区与Fc的融合蛋白，发现其可通过阻断TIM-1/CD4+ T细胞通路减轻肾脏缺血再灌注损伤，提示重组TIM-1蛋白在器官保护中的临床应用潜力。
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以上文献均聚焦于重组HAVCR1/TIM-1蛋白的功能机制研究，涵盖免疫调控、病毒感染和疾病治疗等领域。如需具体期刊信息或发表年份，可进一步补充关键词或研究背景。
Havcr1 (Hepatitis A virus cellular receptor 1), also known as TIM-1 (T-cell immunoglobulin and mucin domain-containing protein 1), is a transmembrane protein belonging to the TIM gene family. It was initially identified as a receptor for hepatitis A virus (HAV) but has since been recognized for its broader roles in immune regulation, particularly in modulating T-cell responses and interactions with apoptotic cells. Structurally, Havcr1 contains an immunoglobulin variable (IgV)-like domain, a mucin-like stalk, a transmembrane region, and a cytoplasmic tail with signaling motifs. The IgV domain mediates binding to phosphatidylserine (PS) exposed on apoptotic cells, enabling Havcr1 to participate in the clearance of cellular debris—a process critical for maintaining tissue homeostasis.
In immunity, Havcr1 is expressed on activated T cells, dendritic cells, and epithelial cells. It influences Th2-type immune responses, allergy, asthma, and autoimmune diseases by regulating cytokine production and cell survival. Studies also link Havcr1 to kidney injury, as its ectodomain is shed during acute tubular damage, making it a potential biomarker for renal disorders. Additionally, Havcr1 interacts with pathogens beyond HAV, including Ebola and Dengue viruses, suggesting a conserved mechanism for viral entry or immune evasion.
Recombinant Havcr1 protein, produced via engineered expression systems (e.g., HEK293 or E. coli), retains functional domains for in vitro studies. It is widely used to investigate ligand-receptor interactions, immune modulation mechanisms, and therapeutic targeting. For example, blocking Havcr1-PS interactions with recombinant proteins has shown promise in mitigating autoimmune inflammation or enhancing antiviral immunity. Its dual role in promoting both pro-inflammatory and anti-inflammatory pathways underscores its therapeutic complexity, driving ongoing research into context-specific targeting strategies. Overall, Havcr1 recombinant proteins serve as vital tools for dissecting immune regulation and developing treatments for infectious, autoimmune, and kidney diseases.
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