| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DRB1 |
| Uniprot No | D7RIG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-266aa |
| 氨基酸序列 | GDTQPRFLWQGKYKCHFFNGTERVQFLERLFYNQEEFVRFDSDVGEYRAVTELGRPVAESWNSQKDILEDRRGQVDTVCRHNYGVGESFTVQRRVHPEVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVMSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS&IKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGVRKSNAAERRGPL |
| 预测分子量 | 28.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and Purification of Recombinant HLA-DRB1 Proteins for Antigen Presentation Studies"** by Smith A, et al.
- 摘要：该文献描述了利用哺乳动物表达系统高效表达HLA-DRB1重组蛋白的方法，并验证其与抗原肽结合及T细胞活化的功能，为研究自身免疫疾病机制提供工具。
2. **"Structural Analysis of HLA-DRB1*04:01 Recombinant Protein in Rheumatoid Arthritis"** by Jones B, et al.
- 摘要：通过X射线晶体学解析HLA-DRB1*04:01重组蛋白的三维结构，揭示其与类风湿性关节炎相关抗原肽的结合特征，支持遗传关联的分子机制。
3. **"Functional Characterization of HLA-DRB1 Recombinant Variants in Multiple Sclerosis"** by Lee C, et al.
- 摘要：利用昆虫细胞表达系统制备不同HLA-DRB1等位基因重组蛋白，分析其与髓鞘抗原的亲和力差异，探讨多发性硬化症的易感性关联。
4. **"HLA-DRB1 Recombinant Protein as a Diagnostic Tool for Celiac Disease"** by Zhang D, et al.
- 摘要：开发基于HLA-DRB1重组蛋白的体外抗原呈递实验，用于检测乳糜泻患者T细胞对麸质肽的特异性反应，提升诊断准确性。
(注：以上文献信息为模拟生成，实际引用需通过学术数据库检索核实。)
**Background of HLA-DRB1 Recombinant Protein**
The HLA-DRB1 gene, part of the human leukocyte antigen (HLA) complex on chromosome 6p21. encodes the β-chain of the HLA-DR heterodimer, a critical component of the MHC class II molecule. HLA-DR molecules are essential for adaptive immunity, presenting exogenous antigenic peptides to CD4+ T cells to initiate immune responses. HLA-DRB1 exhibits remarkable polymorphism, with over 1.000 alleles identified, influencing disease susceptibility, transplant compatibility, and autoimmune risk (e.g., rheumatoid arthritis, multiple sclerosis).
Recombinant HLA-DRB1 proteins are engineered in vitro using expression systems (e.g., mammalian, bacterial, or insect cells) to produce soluble, functional forms of the β-chain. These proteins often include tags (e.g., His-tag) for purification and may be co-expressed with HLA-DRA (α-chain) to form intact HLA-DR complexes. Structural studies confirm their ability to bind antigenic peptides within a conserved groove, with allelic variations dictating peptide-binding specificity.
In research, recombinant HLA-DRB1 proteins serve as tools to study peptide-MHC interactions, T cell receptor recognition, and autoimmune mechanisms. They are also used in epitope mapping, vaccine design, and diagnostics to assess HLA-associated disease risks. Additionally, they aid in developing HLA-targeted therapies, such as blocking antibodies or peptide inhibitors, to modulate aberrant immune responses.
The production of these recombinant proteins addresses challenges in studying native MHC II complexes, which are membrane-bound and difficult to isolate. Their application enhances understanding of HLA-driven immunology and paves the way for personalized therapeutic strategies.
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