| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | sph2 |
| Uniprot No | P59116 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 氨基酸序列 | MINKITKPKLLIGYYLLLFSLIRCLPEKESSYKDLFTSLLFLPNQTNSNQVNSVSINNDPANPNPVNPASANNNQVNAVPENDDPANLNPVNPASANSNQVNAAPENGSPADPNPANLASANNNQVNAVPANNYFTKEDSSNNIPKKVNSKNVEIKVLSHNVFMLPTNLPRWGNLGHDERAKRISKSDYVKNQDVIVFEEAFDTSARKILLDNLREEYPYQTDVVGRTKKNWDASLGNFRSYSLVNGGVVILSKWPIEEKIQYIFNDSGCGADWFANKGFVYVKINKEGKKFHVIGTHAQSQDQNCSNLGIPNRANQFDDIRNFIYSKNIPKDETVLIVGDLNVIKESNEYYDMISRLNVNEPRYVGVPFTWDAKTNEIAAYYYENEEPVYLDYIFVSKSHAQPPVWQNLAYDPVSKQTWTVSGYTSDEFSDHYPIYGFVYADPSTPTKSGHKKKYDQVSFQSAANGKYIQADPNRKNGWLKADAVIETDFTKFNLLQEGNLNPSCIKNGLVRIESSRFLNYFWNWWLGGGSGNYGYYSKFNDASNQLEIINLSDECLENGSKIVFKDYDTYSRNHYYLTVWDKGNWNEHLYLWKDSISQREIFYLKLNSTPVRNWSADLIYR |
| 预测分子量 | 71kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPHK2(鞘氨醇激酶2)重组蛋白的3篇参考文献摘要，供参考：
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1. **文献名称**：*Recombinant human sphingosine kinase 2: production, characterization, and inhibition*
**作者**：Paugh, S.W. et al.
**摘要**：研究报道了人源SPHK2重组蛋白在大肠杆菌中的高效表达与纯化方法，并分析其酶动力学特性。通过体外实验验证了其催化鞘氨醇生成鞘氨醇-1-磷酸(S1P)的功能，并筛选出小分子抑制剂，为靶向SPHK2的疾病治疗提供依据。
2. **文献名称**：*Functional characterization of sphingosine kinase 2 in inflammatory responses using recombinant protein*
**作者**：Liu, H. et al.
**摘要**：利用重组SPHK2蛋白探讨其在巨噬细胞炎症反应中的作用，发现其通过调控S1P水平影响NF-κB信号通路，提示SPHK2可能成为炎症性疾病的潜在治疗靶点。
3. **文献名称**：*Structural insights into the catalytic mechanism of sphingosine kinase 2 through recombinant expression and crystallography*
**作者**：Wang, Q. & Rosen, H.
**摘要**：通过重组SPHK2蛋白的晶体结构解析，揭示了其底物结合口袋和催化活性位点的关键氨基酸残基，为设计特异性抑制剂奠定结构基础。
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注：以上内容基于领域内典型研究方向模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文章，建议以“SPHK2 recombinant protein”为关键词进行精准查询。
**Background of SARS-CoV-2 Spike (S) Recombinant Protein**
The SARS-CoV-2 Spike (S) recombinant protein is a critical component in studying and combating COVID-19. Derived from the surface of the SARS-CoV-2 virus, the Spike protein mediates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor. Structurally, it is a trimeric glycoprotein divided into two functional subunits: S1. responsible for receptor binding, and S2. which drives membrane fusion. The receptor-binding domain (RBD) within S1 is a major target for neutralizing antibodies and vaccine development.
Recombinant Spike proteins are engineered using expression systems like mammalian cells (e.g., HEK293 or CHO) to ensure proper folding and post-translational modifications, such as glycosylation, which are vital for maintaining antigenic authenticity. These proteins are widely utilized in serological assays to detect anti-SARS-CoV-2 antibodies, in vaccine development (e.g., mRNA vaccines encoding Spike), and in research to characterize viral neutralization mechanisms.
The emergence of variants with Spike mutations (e.g., Delta, Omicron) has underscored the need for recombinant Spike proteins to evaluate immune escape and therapeutic efficacy. Additionally, they serve as tools for drug screening, structural studies, and the development of pseudovirus systems for safer viral entry research.
In summary, SARS-CoV-2 Spike recombinant proteins are indispensable for understanding viral pathogenesis, advancing diagnostics, and accelerating the development of vaccines and therapeutics in the ongoing fight against COVID-19.
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