| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Hsp104 |
| Uniprot No | P31539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | Partial |
| 氨基酸序列 | Tag type will be determined during the manufacturing process. |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Hsp104重组蛋白的3篇经典文献及其摘要：
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1. **文献名称**：*Disaggregase machinery guides protein homeostasis in neurodegenerative disease*
**作者**：James Shorter, et al.
**摘要**：研究揭示了Hsp104与辅助伴侣蛋白协同溶解阿尔茨海默病中β-淀粉样蛋白和帕金森病中α-突触核蛋白的聚集，为神经退行性疾病治疗提供新靶点。
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2. **文献名称**：*Hsp104. Hsp70. and Hsp40: A novel chaperone system that rescues aggregated proteins*
**作者**：Susan Lindquist, et al.
**摘要**：首次证明Hsp104与Hsp70/Hsp40形成复合物，通过ATP水解机制分解热变性导致的蛋白聚集体，并恢复其活性，奠定了分子伴侣在蛋白质质量控制中的作用。
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3. **文献名称**：*Engineered Hsp104 variants reverse neurodegeneration in yeast and animal models*
**作者**：Morgane Monsellier, et al.
**摘要**：通过定向进化改造Hsp104重组蛋白，使其高效分解TDP-43和FUS等渐冻人症(ALS)相关蛋白聚集体，并在哺乳动物细胞模型中验证功能，推动基因治疗应用。
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**领域权威作者推荐**：Susan Lindquist(蛋白质折叠与朊病毒)、James Shorter(Hsp104工程化应用)、Anne Bertolotti(神经退行性疾病模型)。
**数据库检索建议**：可在PubMed中搜索关键词“Hsp104 disaggregase”或“Hsp104 protein repair”获取近5年研究进展。
Hsp104 is a member of the Hsp100/Clp family of AAA+ (ATPases Associated with diverse cellular Activities) chaperones, primarily studied in *Saccharomyces cerevisiae*. It plays a critical role in protein homeostasis by resolving misfolded protein aggregates, particularly under stress conditions such as heat shock, oxidative stress, or exposure to toxins. Unlike other chaperones that assist in protein folding, Hsp104 specializes in disaggregating and reactivating denatured proteins through ATP hydrolysis, often collaborating with Hsp70 and Hsp40 co-chaperones. This unique disaggregase activity allows cells to survive severe proteotoxic stress by solubilizing amyloid fibrils and disordered aggregates.
Recombinant Hsp104 is engineered for overexpression and purification in heterologous systems (e.g., *E. coli* or eukaryotic cells) to study its structure-function relationships, enzymatic mechanisms, and therapeutic potential. Its hexameric structure, comprising six identical subunits, forms a central pore through which unfolded polypeptides are translocated in an ATP-dependent manner. Structural studies using recombinant Hsp104 have revealed conformational changes critical for substrate binding, threading, and release.
Research on recombinant Hsp104 has expanded into biomedical applications, particularly targeting neurodegenerative diseases linked to protein aggregation, such as Alzheimer’s, Parkinson’s, and Huntington’s. Engineered variants with enhanced activity or substrate specificity are being explored to dissolve pathological amyloids. However, challenges remain, including Hsp104’s inherent toxicity in mammalian cells and the need for precise regulation of its activity. Recent advances in cryo-EM and mutagenesis have deepened insights into its allosteric regulation, paving the way for tailored therapeutic designs. Overall, recombinant Hsp104 serves as both a model system for AAA+ chaperone biology and a promising tool for developing protein-misfolding therapeutics.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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