| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGLL1 |
| Uniprot No | P15814 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-213aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSLLRPTAA SQSRALGPGA PGGSSRSSLR SRWGRFLLQR GSWTGPRCWP RGFQSKHNSV THVFGSGTQL TVLSQPKATP SVTLFPPSSE ELQANKATLV CLMNDFYPGI LTVTWKADGT PITQGVEMTT PSKQSNNKYA ASSYLSLTPE QWRSRRSYSC QVMHEGSTVE KTVAPAECS |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGLL1重组蛋白的3篇参考文献概览(文献标题和作者为虚构示例，供参考)：
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1. **文献名称**：*Recombinant IGLL1 Protein Expression in Prokaryotic Systems and Its Role in B-Cell Development*
**作者**：Chen L, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组IGLL1蛋白，验证了其在体外促进前B细胞受体组装的功能，揭示了IGLL1在B细胞早期发育中的关键作用。
2. **文献名称**：*Structural Characterization of Human IGLL1 Using Recombinant Protein Technology*
**作者**：Smith J, et al.
**摘要**：通过哺乳动物细胞系统表达重组IGLL1蛋白，结合X射线晶体学解析其三维结构，发现其与免疫球蛋白轻链的相似性，为研究其与配体相互作用提供了结构基础。
3. **文献名称**：*Functional Analysis of IGLL1 in Autoimmune Disorders via Recombinant Protein Knockdown Models*
**作者**：Wang Y, et al.
**摘要**：利用重组IGLL1蛋白制备抗体，构建基因敲低模型，证明IGLL1表达缺失导致B细胞成熟障碍，提示其与某些B细胞相关自身免疫疾病的潜在关联。
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**注**：以上文献信息为示例性内容，实际研究中请通过PubMed、Google Scholar等平台以关键词“IGLL1 recombinant protein”或“IGLL1 expression”检索真实文献。
**Background of IGLL1 Recombinant Protein**
IGLL1 (Immunoglobulin Lambda-Like Polypeptide 1), also known as λ5 or CD179B, is a critical component of the pre-B cell receptor (pre-BCR) complex, essential for early B cell development. It belongs to the immunoglobulin superfamily and functions as a surrogate light chain, pairing with the VpreB protein (CD179A) to form a heterodimer. This complex combines with the μ heavy chain (IgM) on the surface of developing B cell precursors, forming the pre-BCR. Signaling through this receptor promotes survival, proliferation, and differentiation of pro-B cells into pre-B cells, a key step in V(D)J recombination and B cell receptor (BCR) diversification.
IGLL1 is predominantly expressed in the bone marrow during B lymphopoiesis. Mutations or deficiencies in IGLL1 are linked to severe immunodeficiencies, such as agammaglobulinemia, characterized by a near absence of mature B cells and antibodies. Its role extends beyond development; dysregulation of IGLL1 has been implicated in B cell malignancies, including certain leukemias and lymphomas.
Recombinant IGLL1 protein is produced using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to enable functional studies. Purified IGLL1 retains structural and biochemical properties of the native protein, allowing researchers to investigate its interactions with pre-BCR components, signaling pathways (e.g., SYK, BTK), and its role in B cell maturation. It also serves as a tool for developing therapeutic strategies targeting B cell disorders or engineering synthetic B cell models.
Overall, IGLL1 recombinant protein is vital for dissecting B cell biology and advancing immunology research, with translational implications in immunodeficiency and cancer therapeutics.
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