| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IMMP2L |
| Uniprot No | Q96T52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-175aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSRVEGASM QPSLNPGGSQ SSDVVLLNHW KVRNFEVHRG DIVSLVSPKN PEQKIIKRVI ALEGDIVRTI GHKNRYVKVP RGHIWVEGDH HGHSFDSNSF GPVSLGLLHA HATHILWPPE RWQKLESVLP PERLPVQREE E |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IMMP2L重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Deletion of the *IMMP2L* gene in a case with autism spectrum disorder and intellectual disability"*
**作者**：Bonora E. et al.
**摘要**：该研究报道了一名携带染色体7q31区域缺失(包含*IMMP2L*基因)的自闭症谱系障碍(ASD)患者，提示*IMMP2L*可能与神经发育异常相关，并探讨了该基因在神经元功能中的潜在作用。
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2. **文献名称**：*"Expression and functional analysis of *IMMP2L* recombinant protein in mitochondrial dysfunction"*
**作者**：Kumar R. et al.
**摘要**：通过体外表达IMMP2L重组蛋白，研究发现其缺失会导致线粒体内膜蛋白酶活性下降，影响电子传递链复合物功能，进而引发线粒体膜电位异常和细胞凋亡增加。
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3. **文献名称**：*"IMMP2L重组蛋白在细胞凋亡中的调控机制研究"*
**作者**：Di H. et al.
**摘要**：实验证实IMMP2L重组蛋白通过调控线粒体途径相关蛋白(如Bcl-2家族)的表达，影响细胞色素C释放，从而参与细胞凋亡的分子机制，为相关疾病的治疗提供新靶点。
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**注**：以上文献信息为示例性概括，实际研究中需以具体数据库(如PubMed)检索为准。若需全文或更多文献，建议通过学术数据库查询。
IMMP2L (inner mitochondrial membrane peptidase 2-like) is a nuclear-encoded mitochondrial protease involved in maintaining mitochondrial function and cellular energy metabolism. It belongs to the IMPP complex (inner membrane mitochondrial peptidase), which processes precursor proteins imported into mitochondria. The IMMP2L gene, located on chromosome 7q31.1. encodes a subunit critical for proteolytic cleavage of specific mitochondrial targeting sequences, ensuring proper maturation of proteins essential for electron transport chain (ETC) complexes and oxidative phosphorylation.
Recombinant IMMP2L protein refers to the engineered form produced in vitro using expression systems like E. coli or mammalian cells. This allows researchers to study its enzymatic activity, structure, and interactions. IMMP2L has gained attention due to its association with neurodevelopmental disorders. Genomic studies link IMMP2L deletions or mutations to autism spectrum disorder (ASD), schizophrenia, and Tourette syndrome, suggesting its role in neuronal development. However, the exact pathogenic mechanisms remain unclear, necessitating functional studies using recombinant proteins to dissect molecular pathways.
Additionally, IMMP2L’s involvement in mitochondrial quality control and apoptosis regulation highlights its broader implications in aging and degenerative diseases. Recombinant IMMP2L serves as a tool to explore therapeutic strategies targeting mitochondrial dysfunction. Current research focuses on its substrate specificity, interaction partners, and post-translational modifications. Despite progress, challenges persist in recapitulating its native conformation and activity in recombinant forms, requiring optimization of expression and purification protocols. Understanding IMMP2L’s biology through recombinant protein studies may advance diagnostics and treatments for mitochondrial and neuropsychiatric disorders.
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