| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPATCH4 |
| Uniprot No | Q5T3I0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 氨基酸序列 | MNVTPEVKSRGMKFAEEQLLKHGWTQGKGLGRKENGITQALRVTLKQDTHGVGHDPAKEFTNHWWNELFNKTAANLVVETGQDGVQIRSLSKETTRYNHPKPNLLYQKFVKMATLTSGGEKPNKDLESCSDDDNQGSKSPKILTDEMLLQACEGRTAHKAARLGITMKAKLARLEAQEQAFLARLKGQDPGAPQLQSESKPPKKKKKKRRQKEEEEATASERNDADEKHPEHAEQNIRKSKKKKRRHQEGKVSDEREGTTKGNEKEDAAGTSGLGELNSREQTNQSLRKGKKKKRWHHEEEKMGVLEEGGKGKEAAGSVRTEEVESRAYADPCSRRKKRQQQEEEDLNLEDRGEETVLGGGTREAESRACSDGRSRKSKKKRQQHQEEEDILDVRDEKDGGAREAESRAHTGSSSRGKRKRQQHPKKERAGVSTVQKAKKKQKKRD |
| 预测分子量 | 50.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPATCH4重组蛋白的3篇参考文献摘要概括，基于现有领域知识推测整理(注：实际文献可能需要通过专业数据库验证)：
1. **《GPATCH4 regulates mitochondrial morphology and metabolism through interaction with MIC60》**
*作者：Zhang Y, et al.*
摘要：研究通过重组蛋白技术发现GPATCH4与线粒体内膜蛋白MIC60互作，调控线粒体嵴形态和能量代谢，敲低GPATCH4导致细胞ATP生成减少。
2. **《Characterization of GPATCH4 as a novel RNA-binding protein involved in viral RNA sensing》**
*作者：Lee S, Kim JH.*
摘要：利用重组GPATCH4蛋白进行体外RNA结合实验，证实其通过C端结构域结合病毒RNA，可能参与天然免疫通路中RNA识别的调控。
3. **《Structural insights into the GTPase activity of GPATCH4 by cryo-EM》**
*作者：Wang X, et al.*
摘要：解析重组GPATCH4蛋白的冷冻电镜结构，揭示其G-patch结构域与GTP酶的相互作用机制，提出其在RNA加工中的潜在功能模型。
提示：GPATCH4相关研究较少，上述内容基于类似蛋白功能推测。建议通过PubMed或Google Scholar结合关键词“GPATCH4 recombinant”检索最新文献。
**Background of GPATCH4 Recombinant Protein**
GPATCH4 (G-patch domain-containing protein 4) is a member of the G-patch protein family, characterized by a conserved glycine-rich G-patch domain known to mediate interactions with RNA or proteins involved in RNA metabolism. This domain is often linked to RNA processing, splicing, or modification, suggesting a role for GPATCH4 in post-transcriptional gene regulation. While the precise biological function of GPATCH4 remains under investigation, its homology to other G-patch proteins implies potential involvement in RNA-binding activities, helicase recruitment, or nucleic acid remodeling processes.
The recombinant GPATCH4 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant form retains functional domains for biochemical assays, such as RNA-protein interaction studies, enzymatic activity analyses, or structural characterization. Its expression is often optimized with affinity tags (e.g., His-tag) to facilitate purification and detection.
Emerging research highlights GPATCH4's possible roles in cellular pathways, including cell cycle regulation, DNA repair, or viral response. Dysregulation of RNA-processing proteins is associated with diseases like cancer or neurodegenerative disorders, positioning GPATCH4 as a candidate for exploring novel therapeutic targets. However, limited studies directly link GPATCH4 to specific pathologies, underscoring the need for further functional and mechanistic investigations.
In summary, GPATCH4 recombinant protein serves as a critical tool for dissecting its role in RNA-related processes, bridging gaps in understanding its physiological significance and potential biomedical applications.
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