| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DYR; DHFRP1 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DHFR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DHFR抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*Development of a High-Specificity Monoclonal Antibody for Human DHFR Detection*
**作者**：Chen L., et al.
**摘要**：研究团队开发了一种新型单克隆抗体，针对人源DHFR蛋白的C端结构域，验证了其在Western blot和免疫组化中的高灵敏度和低交叉反应性，适用于癌症组织中DHFR表达的定量分析。
2. **文献名称**：*DHFR Overexpression in Methotrexate-Resistant Leukemia Cells: Insights from Antibody-Based Profiling*
**作者**：Smith J.R., Patel K.
**摘要**：通过DHFR特异性抗体检测，揭示了急性淋巴细胞白血病细胞在甲氨蝶呤耐药过程中DHFR蛋白表达显著上调，提示其作为耐药性生物标志物的潜力。
3. **文献名称**：*Comparative Analysis of DHFR Antibody Performance in Immunofluorescence Staining*
**作者**：Wang Y., et al.
**摘要**：系统比较了5种市售DHFR抗体的性能，发现克隆号4F2的抗体在细胞免疫荧光中表现出最优的亚细胞定位特异性，为相关研究提供了抗体选择依据。
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注：以上文献信息为模拟生成，实际引用时请以真实文献为准。建议通过PubMed或Web of Science搜索关键词“DHFR antibody”获取最新研究。
Dihydrofolate reductase (DHFR) is a critical enzyme in cellular metabolism, catalyzing the reduction of dihydrofolate to tetrahydrofolate (THF), a vital cofactor in nucleotide synthesis and amino acid metabolism. DHFR plays a central role in DNA replication, cell proliferation, and one-carbon transfer reactions, making it a therapeutic target for cancer, autoimmune diseases, and infections. DHFR inhibitors, such as methotrexate and trimethoprim, are widely used as chemotherapeutic agents or antimicrobials.
DHFR antibodies are essential tools in biomedical research for detecting and quantifying DHFR expression. They are widely employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study DHFR regulation in drug resistance, cancer progression, or folate metabolism disorders. For example, DHFR upregulation is associated with resistance to methotrexate in cancer cells, and antibodies help identify such mechanisms.
These antibodies are typically generated using recombinant DHFR proteins or peptide antigens, with monoclonal antibodies offering high specificity and polyclonal antibodies providing broader epitope recognition. Researchers also use DHFR antibodies to explore gene expression regulation, protein interactions, and subcellular localization. Commercial DHFR antibodies are often validated for cross-reactivity across species (human, mouse, rat) to support diverse experimental models. Their applications extend to drug development, biomarker discovery, and understanding folate-dependent pathways in diseases like neural tube defects or inflammatory conditions.
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