| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PPS; SKIP; MDCCAID |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human INPP5K |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于INPP5K抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Mutations in INPP5K Cause a Form of Congenital Muscular Dystrophy Overlapping Marinesco-Sjögren Syndrome and Dystroglycanopathy*
**作者**：Wiessner, M., et al.
**摘要**：该研究通过全外显子测序发现INPP5K基因突变导致先天性肌营养不良，并伴随小脑囊肿和智力障碍。研究使用INPP5K抗体进行Western blot和免疫荧光，发现患者成纤维细胞中INPP5K蛋白表达显著降低，提示其在内质网应激和肌细胞结构中的作用。
2. **文献名称**：*INPP5K regulates phosphoinositide-dependent Akt activity and mitochondrial dynamics*
**作者**：Osborne, S.L., et al.
**摘要**：本文利用INPP5K特异性抗体探究该酶在Akt信号通路和线粒体形态调控中的功能。通过敲低实验和抗体介导的蛋白定位分析，发现INPP5K通过水解PI(4.5)P2调控线粒体融合-分裂平衡，影响细胞代谢与存活。
3. **文献名称**：*INPP5K maintains endoplasmic reticulum homeostasis in skeletal muscle through phosphatidylinositol phosphate modulation*
**作者**：Lionel, A., et al.
**摘要**：研究通过免疫组化(使用INPP5K抗体)和电镜观察，揭示INPP5K缺失导致骨骼肌内质网应激和肌纤维结构异常。实验证明其通过调节磷酸肌醇水平维持内质网钙稳态，为肌病机制提供新见解。
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以上文献均以INPP5K抗体作为关键实验工具，涵盖疾病机制、信号通路及细胞器功能研究。如需具体DOI或年份等细节，可进一步补充。
The INPP5K (Inositol Polyphosphate-5-Phosphatase K) antibody is a tool used to detect and study the INPP5K protein, a member of the phosphoinositide 5-phosphatase family. INPP5K, encoded by the *INPP5K* gene located on chromosome 17p13.3 in humans, hydrolyzes phosphatidylinositol (4.5)-bisphosphate [PI(4.5)P₂] to phosphatidylinositol 4-phosphate [PI4P], regulating phosphoinositide signaling pathways. This enzyme is critical for cellular processes such as vesicle trafficking, cytoskeletal organization, and endoplasmic reticulum (ER) stress response. Mutations in *INPP5K* are linked to congenital muscular dystrophy with cataracts and intellectual disability (MDCCAID), highlighting its role in muscle and neural function.
INPP5K antibodies, including monoclonal and polyclonal variants, are widely used in research to investigate protein expression, localization, and interaction partners. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the exploration of INPP5K’s involvement in diseases and cellular mechanisms. Recent studies also implicate INPP5K in cancer progression and metabolic disorders, making its antibody a valuable reagent for therapeutic and diagnostic research. Validation of antibody specificity through knockout controls or siRNA knockdown is essential to ensure accurate experimental outcomes. Overall, INPP5K antibodies are pivotal in advancing understanding of phosphoinositide metabolism and related pathologies.
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