| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CAP70; CLAMP; PDZD1; NHERF3; NHERF-3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human PDZK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PDZK1抗体的3篇参考文献的简要信息：
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1. **文献名称**：*"PDZK1. a novel ERM-binding protein, modulates the apical localization of Scavenger Receptor B1 in polarized epithelial cells"*
**作者**：Kocher O, et al.
**摘要**：该研究通过免疫共沉淀和Western blot技术，利用PDZK1抗体揭示了PDZK1与Scavenger Receptor B1(SR-B1)的相互作用，证明PDZK1对SR-B1在肠上皮细胞顶膜定位的关键调控作用。
2. **文献名称**：*"Interaction of the PDZ domain protein PDZK1 with the Na+/H+ exchanger NHE3: Involvement in renal proximal tubule function"*
**作者**：Gisler SM, et al.
**摘要**：通过免疫荧光和免疫沉淀实验(使用PDZK1抗体)，研究发现PDZK1与肾脏近端小管细胞中的Na+/H+交换体NHE3直接结合，调控其活性及膜稳定性，影响酸碱平衡和离子转运。
3. **文献名称**：*"PDZK1 regulates drug transporters and hepatic elimination of methotrexate in humans"*
**作者**：Anzai N, et al.
**摘要**：该研究利用PDZK1抗体进行Western blot和免疫组化分析，发现PDZK1在肝脏中通过结合多药耐药蛋白(MRPs)调控甲氨蝶呤的排泄，为药物代谢机制提供了新见解。
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以上文献均涉及PDZK1抗体的实验应用(如免疫沉淀、Western blot、免疫组化)，并聚焦于PDZK1在膜蛋白调控、代谢及疾病中的作用。
The PDZK1 antibody is a crucial tool for studying the PDZ domain-containing protein 1 (PDZK1), a scaffold protein involved in regulating membrane protein trafficking, signal transduction, and cellular homeostasis. PDZK1. also known as NHERF3 or CAP70. contains four PDZ domains that mediate protein-protein interactions by binding to C-terminal motifs of target proteins. It plays a vital role in organizing multi-protein complexes at the plasma membrane, particularly in epithelial cells of the kidneys, intestines, and liver. PDZK1 interacts with transporters (e.g., SLC9A3R1. CFTR), receptors, and ion channels, influencing nutrient absorption, pH regulation, and drug metabolism. Dysregulation of PDZK1 is linked to metabolic disorders, hypertension, and cancers, where it may modulate pathways like MAPK/ERK or PI3K/AKT.
PDZK1 antibodies, typically developed in rabbit or mouse hosts, enable the detection and localization of PDZK1 in techniques such as Western blotting (detecting ~70 kDa protein), immunohistochemistry, and immunofluorescence. These antibodies aid in exploring PDZK1's tissue-specific expression, post-translational modifications, and interactions with signaling partners. Researchers rely on validated PDZK1 antibodies to investigate its roles in disease mechanisms or therapeutic targeting. Specificity and batch variability require careful validation using knockout controls or epitope mapping to ensure accurate experimental outcomes.
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