| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NIN283 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ZNRF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于ZNRF1抗体的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*ZNRF1 regulates ubiquitination and degradation of synaptic proteins in neuronal development*
**作者**：Li, X., et al.
**摘要**：本研究揭示了ZNRF1作为E3泛素连接酶在神经元突触蛋白降解中的作用，利用特异性ZNRF1抗体验证其与突触素(synaptophysin)的相互作用，并证明其缺失导致突触功能异常。
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2. **文献名称**：*Structural basis of ZNRF1 recognition by ubiquitin-conjugating enzymes*
**作者**：Wang, Y., et al.
**摘要**：通过冷冻电镜解析ZNRF1与E2酶复合物的结构，研究使用ZNRF1抗体进行免疫共沉淀实验，阐明了其底物结合域的功能机制，为靶向泛素化通路的药物开发提供依据。
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3. **文献名称**：*ZNRF1 suppresses tumor growth by targeting oncogenic receptors for degradation*
**作者**：Chen, Z., et al.
**摘要**：发现ZNRF1在多种癌症中表达下调，通过ZNRF1抗体检测发现其通过泛素化降解EGFR等受体酪氨酸激酶，抑制肿瘤增殖，提示其作为潜在抑癌因子的作用。
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4. **文献名称**：*Antibody validation and functional analysis of ZNRF1 in mouse models of neurodegeneration*
**作者**：Smith, J., et al.
**摘要**：验证了多种ZNRF1抗体的特异性，并利用小鼠模型证明ZNRF1在阿尔茨海默病中通过调控Tau蛋白泛素化影响神经元存活，为疾病机制研究提供工具支持。
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**注意**：以上文献为模拟示例，实际研究需通过PubMed、Google Scholar或Web of Science等平台检索关键词“ZNRF1 antibody”或“ZNRF1 ubiquitin ligase”获取。
The ZNRF1 (Zinc and Ring Finger 1) antibody is a research tool used to detect and study the ZNRF1 protein, an E3 ubiquitin ligase involved in regulating cellular processes such as protein degradation, membrane receptor trafficking, and signaling pathways. ZNRF1 contains a characteristic RING finger domain, enabling its role in ubiquitination—a post-translational modification that tags proteins for proteasomal degradation or functional modulation. It is particularly implicated in the Wnt signaling pathway by targeting Frizzled receptors for lysosomal degradation, thus acting as a negative regulator of Wnt/β-catenin signaling, which is critical in development, stem cell maintenance, and cancer progression.
Studies highlight ZNRF1's involvement in neuronal development, cancer biology, and metabolic regulation. Dysregulation of ZNRF1 has been linked to tumors, neurodegenerative disorders, and metabolic diseases, making it a potential therapeutic target. The ZNRF1 antibody is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interaction partners in cell lines or tissue samples. Researchers also employ it to explore mechanisms underlying ZNRF1-mediated ubiquitination and its crosstalk with pathways like Notch or TGF-β. Available as polyclonal or monoclonal versions, the antibody’s specificity and validation (e.g., knockout validation) are essential for ensuring reliable experimental outcomes in both basic and translational research contexts.
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