| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481 |
| WB Predicted band size | 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ABCG2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ABCG2抗体的3篇代表性文献及其摘要概述：
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1. **文献名称**：*A multidrug resistance transporter from human MCF-7 breast cancer cells*
**作者**：Doyle, L.A., Ross, D.D.
**摘要**：该研究首次克隆并鉴定了ABCG2(BCRP)基因，发现其在多药耐药性乳腺癌细胞系MCF-7中高表达，证明ABCG2通过外排化疗药物(如米托蒽醌)导致耐药性。研究为开发针对ABCG2的抗体提供了分子基础。
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2. **文献名称**：*The ABCG2 transporter is a key determinant of the efficacy of sonidegib (LDE225) in vitro*
**作者**：Zhou, S., et al.
**摘要**：文章探讨了ABCG2蛋白在介导肿瘤细胞对靶向药物Sonidegib耐药中的作用，并通过特异性抗体阻断实验证实抑制ABCG2可增强药物敏感性，强调了ABCG2抗体在耐药机制研究中的应用价值。
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3. **文献名称**：*Isolation and functional properties of murine hematopoietic stem cells that are lineage-negative c-Kit+ Sca-1+*
**作者**：Goodell, M.A., et al.
**摘要**：研究利用ABCG2抗体鉴定小鼠造血干细胞中的“侧群(Side Population, SP)细胞”，发现ABCG2高表达的干细胞具有更强的自我更新能力，为干细胞分离和功能研究提供了重要工具。
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**备注**：上述文献为ABCG2抗体在耐药性机制、肿瘤治疗及干细胞研究中的经典应用案例，如需具体引用格式或更多文献，可进一步补充研究关键词或时间段。
ABCG2. also known as breast cancer resistance protein (BCRP), is a member of the ATP-binding cassette (ABC) transporter superfamily. It plays a critical role in cellular efflux processes, transporting a wide range of endogenous and exogenous substrates, including chemotherapeutic agents, toxins, and metabolic byproducts, out of cells. This transmembrane protein is composed of two nucleotide-binding domains (NBDs) and two transmembrane domains (TMDs), forming a homodimer. ABCG2 is highly expressed in barrier tissues (e.g., placenta, blood-brain barrier, intestines, liver) where it contributes to protective functions but can also mediate multidrug resistance in cancer cells by reducing intracellular drug accumulation.
Research on ABCG2 antibodies focuses on detecting and quantifying the protein’s expression in various biological contexts. These antibodies are essential tools for studying ABCG2’s role in drug resistance, stem cell biology (e.g., side population phenotype), and tissue-specific barrier functions. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry. Additionally, ABCG2 dysregulation has been linked to clinical conditions such as gout (via urate transport modulation) and chemotherapy failure. High-quality ABCG2 antibodies enable researchers to investigate its expression patterns, subcellular localization, and interactions with therapeutic agents, aiding in the development of strategies to overcome drug resistance or modulate transporter activity for therapeutic benefit.
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