| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | FKH5; HFKH-5 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human FOXB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于FOXB1抗体的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库检索确认):
1. **标题**: "FOXB1 regulates neural progenitor differentiation via Wnt signaling"
**作者**: Smith J, et al.
**期刊**: Developmental Biology, 2020
**摘要**: 本研究利用FOXB1特异性抗体进行免疫荧光染色，发现FOXB1在小鼠胚胎脑室区的神经前体细胞核内高表达。通过功能实验证实FOXB1通过调控Wnt通路关键基因维持神经干细胞的增殖-分化平衡。
2. **标题**: "FOXB1 as a novel biomarker in glioblastoma progression"
**作者**: Zhang Y, et al.
**期刊**: Oncogene, 2018
**摘要**: 通过Western blot和免疫组化分析，作者使用兔源FOXB1单克隆抗体发现胶质瘤组织中FOXB1蛋白表达水平与患者生存率呈负相关。机制研究表明FOXB1通过激活EGFR信号通路促进肿瘤侵袭。
3. **标题**: "Genome-wide analysis of FOXB1 binding sites in lung development"
**作者**: Johnson R, et al.
**期刊**: Cell Reports, 2019
**摘要**: 采用ChIP-seq技术结合FOXB1抗体，鉴定出在胚胎肺上皮发育过程中FOXB1直接调控的327个靶基因，包括关键形态发生相关因子Sonic Hedgehog(SHH)，揭示了FOXB1在器官分支形态发生中的核心作用。
4. **标题**: "Antibody validation for FOXB1 in cross-species studies"
**作者**: Müller T, et al.
**期刊**: Scientific Reports, 2021
**摘要**: 系统验证了商用FOXB1抗体(货号AB1234)在小鼠、斑马鱼和人类组织中的交叉反应性，通过CRISPR敲除实验证实抗体特异性，为跨物种比较研究提供了可靠工具。
提示：实际研究中建议通过PubMed或Web of Science以"FOXB1 antibody"+"应用领域关键词"(如immunohistochemistry、ChIP-seq等)组合检索最新文献，并注意检查抗体的货号及物种反应性是否符合实验需求。
FOXB1. a member of the forkhead box (FOX) transcription factor family, is characterized by a conserved forkhead DNA-binding domain that facilitates sequence-specific interactions with target genes. FOX proteins play pivotal roles in embryonic development, tissue homeostasis, and disease pathogenesis. While FOXB1’s functional roles are less extensively studied compared to other FOX members (e.g., FOXO, FOXP3), emerging evidence suggests its involvement in cellular differentiation and organogenesis, particularly in neural and gastrointestinal systems. Dysregulation of FOXB1 has been tentatively linked to developmental anomalies and cancers, though mechanistic insights remain limited.
FOXB1 antibodies are critical tools for investigating its expression, localization, and molecular interactions. These antibodies, often generated in rabbits or mice using immunogenic peptides from conserved regions of FOXB1. enable applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validation typically includes knockout cell lines or siRNA knockdown to confirm specificity. In research, FOXB1 antibodies help elucidate its transcriptional networks, post-translational modifications, and roles in signaling pathways. For instance, studies using these antibodies have explored FOXB1’s potential as a biomarker in certain cancers or its regulatory effects on epithelial-mesenchymal transition (EMT). Despite limited commercial availability, custom FOXB1 antibodies continue to support niche investigations into developmental biology and oncogenesis, underscoring the need for further functional characterization of this understudied transcription factor.
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