| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDOC1 |
| Uniprot No | O95751 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MVDELVLLLH ALLMRHRALS IENSQLMEQL RLLVCERASL LRQVRPPSCP VPFPETFNGE SSRLPEFIVQ TASYMLVNEN RFCNDAMKVA FLISLLTGEA EEWVVPYIEM DSPILGDYRA FLDEMKQCFG WDDDEDDDDE EEEDDY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与LDOC1重组蛋白相关的文献摘要信息(注：作者和文献名为模拟示例，实际文献请通过学术数据库核实)：
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1. **文献名称**：LDOC1 suppresses NF-κB-mediated inflammatory response by interacting with HDAC3
**作者**：Sasaki, T. et al.
**摘要**：本研究通过大肠杆菌表达系统获得重组LDOC1蛋白，发现其通过结合组蛋白去乙酰化酶HDAC3.抑制NF-κB信号通路的活化，从而降低炎症因子IL-6和TNF-α的表达，揭示了LDOC1在免疫调控中的潜在作用。
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2. **文献名称**：Recombinant LDOC1 induces mitochondrial apoptosis in cervical cancer cells
**作者**：Nagahara, H. et al.
**摘要**：作者利用昆虫细胞表达体系纯化LDOC1重组蛋白，发现其可直接诱导线粒体膜电位丧失，激活Caspase-9依赖的凋亡通路，抑制宫颈癌细胞增殖，为LDOC1作为肿瘤治疗靶点提供实验依据。
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3. **文献名称**：Structural characterization of LDOC1 and its interaction with PI3K
**作者**：Mizushima, K. et al.
**摘要**：通过X射线晶体学解析重组LDOC1蛋白的三维结构，发现其亮氨酸拉链结构域是结合PI3K激酶的关键区域，抑制PI3K/Akt信号传导，阐明LDOC1在肿瘤代谢调控中的分子机制。
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如需精准文献，建议在PubMed或Web of Science中检索关键词“LDOC1 recombinant protein”或结合具体研究领域筛选。
LDOC1 (Leucine Zipper, Downregulated in Cancer 1) recombinant protein is derived from the LDOC1 gene, initially identified as a tumor-associated gene with reduced expression in various cancers. Discovered in the late 1990s, LDOC1 encodes a small nuclear protein containing a leucine zipper domain, a structural motif implicated in protein-protein interactions and transcriptional regulation. Though its precise molecular function remains under investigation, LDOC1 is hypothesized to act as a tumor suppressor, influencing apoptosis and cell proliferation through interactions with signaling pathways like NF-κB.
Studies show LDOC1 expression is frequently downregulated in malignancies such as pancreatic, gastric, and cervical cancers, correlating with poor prognosis. This suppression is often linked to promoter hypermethylation or epigenetic modifications. Recombinant LDOC1 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies in vitro. Researchers use it to explore its role in inhibiting cancer cell growth, modulating immune responses, or regulating gene expression networks. Tagged versions (e.g., His-tag) facilitate purification and detection in assays.
Current research focuses on LDOC1's potential as a diagnostic biomarker or therapeutic target. Its recombinant form aids in antibody development, drug screening, and mechanistic studies to clarify its anti-tumor activities. Despite progress, challenges persist in understanding its tissue-specific roles and interactions with oncogenic pathways, highlighting the need for further structural and functional characterization.
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