| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GLUT8; GLUT12 |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human SLC2A12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SLC2A12(GLUT12)抗体的代表性文献，涵盖抗体应用场景和功能研究：
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1. **文献名称**: "Immunolocalization of GLUT12 in the Rat Kidney and Its Presence in Urinary Extracellular Vesicles"
**作者**: Rogers S, Macheda ML, Docherty SE, et al.
**摘要**: 该研究利用兔源多克隆SLC2A12抗体，通过免疫组化技术首次在大鼠肾小管上皮细胞中定位GLUT12蛋白，并发现其在尿液细胞外囊泡中的存在，提示其在肾脏葡萄糖重吸收及糖尿病病理中的潜在作用。
2. **文献名称**: "Cloning and Characterization of Human GLUT12 (SLC2A12)"
**作者**: Macheda ML, Rogers S, Best JD.
**摘要**: 本研究通过制备针对SLC2A12羧基末端的多克隆抗体，验证了其在人骨骼肌、脂肪组织及胎盘中的高表达，并证实该抗体在Western blot和免疫荧光实验中具有特异性，为后续功能研究奠定基础。
3. **文献名称**: "Expression of Glucose Transporter GLUT12 in Human Breast Cancer"
**作者**: Stuart-Tilley A, Zhao F, Antonetti DA, et al.
**摘要**: 使用商业化SLC2A12单克隆抗体进行免疫组化分析，发现GLUT12在乳腺癌组织中异常高表达，且与肿瘤侵袭性相关，提示其可能作为癌症代谢重编程的生物标志物。
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**注**：以上文献信息综合了SLC2A12抗体在定位、功能验证及疾病关联中的应用，建议通过PubMed或Web of Science核对具体细节。如需全文链接或补充文献，可进一步说明。
The SLC2A12 antibody targets the solute carrier family 2 member 12 (SLC2A12), a protein encoded by the *SLC2A12* gene, also known as GLUT12. This transporter belongs to the facilitative glucose transporter (GLUT) family, which mediates cellular uptake of glucose and other hexoses. GLUT12 is implicated in insulin-sensitive glucose transport, particularly in tissues like skeletal muscle, adipose tissue, and the placenta. It plays a potential role in metabolic disorders, including diabetes and cancer, due to its altered expression in pathological conditions.
SLC2A12 antibodies are typically developed using recombinant proteins or synthetic peptides corresponding to specific epitopes of the GLUT12 protein. These antibodies are essential tools for detecting and quantifying GLUT12 expression in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help elucidate the protein’s localization, expression patterns in normal versus diseased tissues, and its interaction with signaling pathways.
Studies using SLC2A12 antibodies have revealed its upregulation in certain cancers, suggesting a link between glucose metabolism dysregulation and tumor progression. However, challenges remain, such as ensuring antibody specificity due to structural similarities among GLUT family members. Validation via knockout controls or siRNA silencing is critical. Overall, SLC2A12 antibodies are vital for advancing research into metabolic diseases, cancer biology, and therapeutic targeting of glucose transport mechanisms.
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