| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MC2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MC5R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MC5R抗体的3篇代表性文献，内容涵盖抗体开发、功能验证及应用研究：
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1. **文献名称**：*Characterization of a polyclonal antibody to the melanocortin 5 receptor*
**作者**：Chen et al.
**摘要**：研究团队开发了一种针对MC5R的多克隆抗体，通过免疫印迹和免疫组化验证其特异性，证实其在多种哺乳动物组织中检测MC5R蛋白表达的可靠性，并发现MC5R在脂肪组织和免疫细胞中高表达。
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2. **文献名称**：*Role of MC5R in sebaceous gland function: Insights from antibody-based localization studies*
**作者**：Garcia et al.
**摘要**：利用MC5R特异性抗体进行免疫荧光定位，揭示MC5R在皮脂腺中的分布，证明其通过调控脂质合成影响皮肤屏障功能，为治疗痤疮等皮肤疾病提供潜在靶点。
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3. **文献名称**：*MC5R antibody blockade ameliorates metabolic dysfunction in obese mouse models*
**作者**：Smith et al.
**摘要**：研究通过抗MC5R抗体抑制受体活性，发现可显著改善肥胖小鼠的胰岛素敏感性和脂肪代谢，提示MC5R在能量平衡中的作用，为代谢综合征治疗提供新思路。
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**备注**：以上文献为示例，实际引用时需根据具体研究领域补充真实文献(可通过PubMed或Web of Science搜索关键词如“MC5R antibody”或“melanocortin 5 receptor antibody”获取最新研究)。
Melanocortin 5 receptor (MC5R) antibody is a research tool designed to detect and study the MC5R protein, a member of the melanocortin receptor family. MC5R is a G protein-coupled receptor (GPCR) that binds α-melanocyte-stimulating hormone (α-MSH) and other melanocortin peptides. It is widely expressed in peripheral tissues, including exocrine glands, adipose tissue, and immune cells, and is implicated in diverse physiological processes such as sebum production, thermoregulation, immune modulation, and energy homeostasis. Dysregulation of MC5R signaling has been linked to metabolic disorders, inflammation, and cancer.
MC5R antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant proteins corresponding to specific epitopes of the receptor. These antibodies enable the detection of MC5R in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in the localization and quantification of the receptor in tissues or cell lines. Validation often includes knockout controls to confirm specificity.
Research utilizing MC5R antibodies has advanced understanding of its role in lipid metabolism, immune responses, and endocrine regulation. They are critical tools for exploring MC5R's therapeutic potential in conditions like obesity, diabetes, and autoimmune diseases. Challenges remain in ensuring antibody specificity due to homology among melanocortin receptors, underscoring the need for rigorous validation in experimental models.
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