| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | bHLHc2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MYF5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为模拟生成的MYF5抗体相关参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "MYF5 expression marks satellite cell activation during muscle regeneration"
**作者**: Smith A, et al.
**摘要**: 通过MYF5抗体免疫荧光染色，揭示了小鼠骨骼肌损伤后卫星细胞中MYF5蛋白的动态表达模式，证实其在肌肉再生中的早期激活作用。
2. **文献名称**: "Comparative analysis of MYF5 and MYOD1 in embryonic myogenesis"
**作者**: Chen L & Rao M.
**摘要**: 利用MYF5抗体进行胚胎组织Western blot及免疫组化，证明MYF5在脊椎动物胚胎肌节形成早于MYOD1.提示其驱动成肌前体细胞定向分化。
3. **文献名称**: "MYF5 antibody validation for flow cytometry in human mesenchymal stem cells"
**作者**: Gupta R, et al.
**摘要**: 系统验证了商业MYF5抗体(货号AB123)在人源间充质干细胞肌源性分化研究中的特异性，优化了流式检测方案并建立定量标准。
4. **文献名称**: "Aberrant MYF5 expression in muscular dystrophy models"
**作者**: Tanaka K, et al.
**摘要**: 通过免疫共沉淀和MYF5抗体染色，发现DMD模型小鼠肌肉组织中MYF5蛋白异常聚集，提示其与肌纤维修复障碍存在相关性。
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建议通过PubMed或Web of Science搜索真实文献，可使用关键词：**MYF5 antibody skeletal muscle** 或 **MYF5 immunohistochemistry validation**。注意核对抗体货号及物种交叉反应性数据。
MYF5 (Myogenic Factor 5) is a member of the myogenic regulatory factors (MRFs), a family of transcription factors critical for skeletal muscle development and differentiation. Discovered in the early 1990s, MYF5 is encoded by the *MYF5* gene and plays a pivotal role in myogenesis, particularly during embryonic development. It is expressed early in the commitment of mesodermal precursor cells to the myogenic lineage, working alongside other MRFs like MYOD, myogenin (MYOG), and MRF4 to regulate muscle-specific gene expression. Structurally, MYF5 contains a basic helix-loop-helix (bHLH) domain essential for DNA binding and dimerization with E-proteins to activate target genes.
Antibodies against MYF5 are widely used in research to study muscle cell differentiation, regeneration, and diseases such as muscular dystrophies or rhabdomyosarcoma. These antibodies enable detection of MYF5 protein in various assays, including immunohistochemistry (IHC), Western blotting, and immunofluorescence, helping to identify myogenic progenitors or satellite cells in tissues. MYF5’s expression is often transient and cell-stage-specific, making its antibodies valuable for tracing developmental pathways. However, functional redundancy with other MRFs and overlapping expression patterns in certain contexts require careful experimental validation. Commercially available MYF5 antibodies are typically raised in rabbits or mice, with specificity confirmed using knockout controls. Studies utilizing MYF5 antibodies have significantly advanced understanding of muscle biology, stem cell therapies, and cancer mechanisms involving myogenic deregulation.
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