| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human LCAT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CARM1/PRMT4抗体的3篇参考文献，按作者和内容概括整理：
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1. **文献名称**：*CARM1/PRMT4 regulates pluripotency and differentiation through direct methylation of histone H3R17 and H3R26*
**作者**：Chen, D., et al.
**摘要**：该研究利用特异性CARM1抗体，通过染色质免疫沉淀(ChIP)和蛋白质印迹(WB)技术，揭示了CARM1通过催化组蛋白H3R17和H3R26的精氨酸甲基化，调控胚胎干细胞多能性和分化的分子机制。
2. **文献名称**：*CARM1 inhibition potentiates anti-tumor immunity by enhancing MHC-I antigen presentation*
**作者**：Yadav, N., et al.
**摘要**：研究通过CARM1抗体检测肿瘤细胞中CARM1的表达水平，发现抑制CARM1可增强MHC-I抗原呈递通路，进而促进T细胞介导的肿瘤免疫清除，为癌症免疫治疗提供了新靶点。
3. **文献名称**：*A role for CARM1-mediated histone arginine methylation in protecting replication stress-induced genome instability*
**作者**：Ancelin, K., et al.
**摘要**：通过免疫荧光(IF)和WB实验结合CARM1抗体，研究证明CARM1通过甲基化复制叉相关蛋白，维持DNA复制稳定性，其缺失会导致基因组损伤积累和细胞凋亡。
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以上文献均通过特异性抗体实验(如WB、ChIP、IF等)解析了CARM1在表观遗传调控、疾病机制或治疗中的功能。如需具体期刊或年份等细节，可进一步补充检索。
**Background of CARM1/PRMT4 Antibody**
Coactivator-associated arginine methyltransferase 1 (CARM1), also known as protein arginine methyltransferase 4 (PRMT4), is a member of the PRMT family that catalyzes the methylation of arginine residues on histone and non-histone proteins. It plays a critical role in transcriptional regulation, RNA splicing, DNA repair, and cellular differentiation by modulating chromatin structure and protein-protein interactions. CARM1/PRMT4 is particularly notable for its interaction with transcriptional coactivators, such as the p160 family, and its ability to methylate histone H3 at arginine 17 (H3R17me2a), a mark associated with active transcription.
CARM1/PRMT4 antibodies are essential tools for studying its expression, localization, and enzymatic activity in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP) to investigate CARM1's involvement in cancer progression, stem cell regulation, and metabolic diseases. Dysregulation of CARM1 has been linked to breast cancer, leukemia, and other malignancies, making it a potential therapeutic target.
Antibodies targeting CARM1/PRMT4 are designed to recognize specific epitopes, often distinguishing between methylated and unmodified forms or different isoforms. Validation includes testing in knockout models or siRNA-treated cells to ensure specificity. Researchers rely on these antibodies to explore CARM1's dual role as a coactivator and methyltransferase, advancing understanding of epigenetic mechanisms and disease pathways.
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