| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TCF-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TCF7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TCF7抗体的3篇文献示例(模拟内容供参考)：
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1. **文献名称**：TCF7 regulates immune checkpoint expression in CD8+ T cells during chronic viral infection
**作者**：Smith A, et al.
**摘要**：通过Western blot和流式细胞术分析，使用TCF7抗体证实TCF7在耗竭性CD8+ T细胞中的表达下调，揭示其通过调控PD-1等免疫检查点分子影响抗病毒T细胞功能。
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2. **文献名称**：TCF7-dependent Wnt signaling promotes colorectal cancer stemness
**作者**：Chen L, et al.
**摘要**：采用TCF7抗体进行免疫组织化学染色，发现TCF7在结直肠癌干细胞中高表达，并通过激活Wnt/β-catenin通路促进肿瘤干性维持和化疗耐药。
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3. **文献名称**：TCF7 controls T follicular helper cell differentiation via epigenetic modulation
**作者**：Wang Y, et al.
**摘要**：利用TCF7抗体进行ChIP-seq和免疫荧光实验，阐明TCF7通过结合Bcl6启动子调控滤泡辅助T细胞分化，影响体液免疫应答。
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(注：以上文献为示例性内容，实际引用需查询具体数据库如PubMed或Google Scholar获取真实文献。)
TCF7 (T-cell factor 7), also known as TCF-1. is a transcription factor encoded by the *TCF7* gene and a key mediator of the Wnt/β-catenin signaling pathway. It belongs to the TCF/LEF (lymphoid enhancer-binding factor) family, which binds to DNA through a high-mobility group (HMG) domain and regulates target gene expression. TCF7 plays a critical role in embryonic development, immune system regulation, and cell fate determination. In the immune context, it is essential for T-cell development and differentiation, particularly in thymocyte maturation and the formation of CD8+ T-cell memory. Dysregulation of TCF7 is implicated in various diseases, including cancers (e.g., colorectal, leukemia) and autoimmune disorders.
TCF7 antibodies are widely used in research to detect and quantify TCF7 protein expression in cells or tissues. They enable studies on its nuclear localization, interaction with β-catenin, and regulatory mechanisms in Wnt-driven processes. Common applications include Western blotting, immunohistochemistry, flow cytometry, and chromatin immunoprecipitation (ChIP). These antibodies help elucidate TCF7's dual role as both an oncogene and tumor suppressor, depending on cellular context and post-translational modifications. Recent interest has focused on its involvement in immune checkpoint regulation and cancer immunotherapy, particularly its association with T-cell exhaustion in tumors. Validated TCF7 antibodies are crucial for advancing research in immunology, oncology, and developmental biology.
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