| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | FMR2; MRX2; OX19; FMR2P; FRAXE |
| WB Predicted band size | 145 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human AFF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与AFF2抗体相关的文献概览(部分文献可能涉及AFF2基因功能或抗体应用)：
1. **"AFF2-FMR2 complex is required for Cbp-dependent histone acetylation and brain development"**
- **作者**: Kim YS, et al.
- **摘要**: 研究通过AFF2抗体进行染色质免疫共沉淀(ChIP)，揭示AFF2蛋白与组蛋白乙酰化酶CBP的相互作用，阐明其在神经元发育及自闭症相关通路中的作用。
2. **"Antibody-based profiling of AFF2 expression in fragile X-associated tremor/ataxia syndrome"**
- **作者**: Hagerman RJ, et al.
- **摘要**: 利用特异性AFF2抗体检测脆性X相关震颤共济失调综合征(FXTAS)患者脑组织中的蛋白表达异常，提示AFF2在神经退行性疾病中的潜在病理机制。
3. **"Characterization of AFF2 isoforms and antibody validation in X-linked intellectual disability models"**
- **作者**: Gecz J, et al.
- **摘要**: 通过Western blot和免疫荧光验证多种AFF2抗体的特异性，分析不同剪接异构体在X连锁智力障碍患者细胞模型中的表达差异。
4. **"Epigenetic regulation by AFF2 in cortical neurons: Insights from knockout mice and CRISPR-Cas9 editing"**
- **作者**: Lee J, et al.
- **摘要**: 研究使用AFF2抗体在基因敲除小鼠中定位蛋白表达，结合表观遗传学分析，揭示AFF2对皮层神经元突触可塑性的调控作用。
注：若需具体文献来源或全文链接，可提供数据库(如PubMed ID)进一步检索。
The AFF2 antibody is a research tool targeting the AFF2 protein (AF4/FMR2 family member 2), also known as FMR2. AFF2 is a member of the AFF family of nuclear transcriptional regulators, which play roles in transcriptional elongation, chromatin remodeling, and RNA-binding processes. The AFF2 gene is located on the X chromosome (Xq28) and is associated with neurodevelopmental disorders. Mutations or epigenetic silencing of AFF2 are linked to FRAXE intellectual disability, a rare X-linked syndrome characterized by cognitive impairment, behavioral challenges, and speech delays. FRAXE is distinct from Fragile X syndrome (FMR1-related) but shares overlapping clinical features.
AFF2 antibodies are primarily used in biomedical research to study protein expression, localization, and function in cellular and disease models. They enable detection of AFF2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies using these antibodies have helped elucidate AFF2's role in neuronal development, synaptic plasticity, and its interaction with other transcriptional regulators. Despite progress, AFF2's precise molecular mechanisms remain incompletely understood, and the antibody serves as a critical reagent for ongoing investigations into neurodevelopmental disorders and potential therapeutic targets. Commercial AFF2 antibodies are typically validated in knockout controls to ensure specificity.
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