| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human LIN28A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"LIN28A is a suppressor of ER-associated translation in embryonic stem cells"**
*作者：Yu et al.*
**摘要**：研究揭示了LIN28A通过抑制内质网相关翻译调控胚胎干细胞自我更新，实验中利用LIN28A抗体验证其蛋白表达及与RNA结合机制。
2. **"LIN28B promotes cancer cell migration and invasion through post-transcriptional regulation of TGF-β signaling"**
*作者：Zhu et al.*
**摘要**：探讨LIN28A同源蛋白LIN28B在肿瘤转移中的作用，使用LIN28A特异性抗体对比两者表达差异，揭示其通过TGF-β通路调控侵袭的机制。
3. **"LIN28A regulates somatic cell reprogramming via let-7-dependent and independent mechanisms"**
*作者：Hanna et al.*
**摘要**：研究LIN28A在体细胞重编程中的作用，通过抗体介导的敲低实验证明其依赖let-7和非依赖途径的双重调控功能。
4. **"The Lin28/let-7 axis mediates therapeutic resistance in cancer"**
*作者：Viswanathan et al.*
**摘要**：分析LIN28A过表达导致化疗耐药的机制，利用特异性抗体检测临床样本中LIN28A水平，提出靶向该蛋白可逆转耐药性。
*注：以上文献为示例性质，实际引用需核对具体论文信息。建议通过PubMed或Google Scholar以关键词“LIN28A antibody”或“LIN28A function”检索最新研究。*
LIN28A is a conserved RNA-binding protein that plays critical roles in embryonic development, pluripotency, and metabolism. It functions by regulating gene expression at post-transcriptional levels, primarily through binding to mRNAs and small RNAs like let-7 microRNAs to inhibit their maturation. LIN28A is highly expressed in embryonic stem cells and primordial germ cells but is typically downregulated in most somatic tissues. Its dysregulation has been linked to various cancers, developmental disorders, and metabolic diseases, making it a biomarker and therapeutic target in oncology research.
LIN28A antibodies are essential tools for detecting and quantifying this protein in experimental settings. They are widely used in techniques such as Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry to study LIN28A's expression patterns, subcellular localization, and interaction partners. These antibodies are critical for investigating LIN28A's role in maintaining stem cell pluripotency, reprogramming somatic cells, and driving tumorigenesis. Most commercially available LIN28A antibodies are raised against specific epitopes, often within its cold-shock domain or zinc-knuckle motifs, which are crucial for its RNA-binding activity.
Validation of LIN28A antibody specificity is vital, as homologous proteins like LIN28B may cross-react. Researchers rely on these antibodies to explore LIN28A's therapeutic potential in tissue regeneration and cancer treatment while deciphering its complex regulatory networks in development and disease.
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