| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | INT3 |
| WB Predicted band size | 210 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NOTCH4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOTCH4抗体的3篇示例文献(注：文献为虚构示例，实际文献请通过学术数据库查询)：
1. **文献名称**: *NOTCH4 Antibody Characterization in Vascular Remodeling Studies*
**作者**: Smith A, et al.
**摘要**: 研究验证了一种新型NOTCH4抗体的特异性，发现其在人内皮细胞中能有效检测NOTCH4蛋白表达。实验表明，NOTCH4通过调控内皮间质转化参与动脉粥样硬化的病理过程。
2. **文献名称**: *Role of NOTCH4 in T-cell Lymphoma: Insights from Antibody-based Inhibition*
**作者**: Chen L, et al.
**摘要**: 通过使用NOTCH4中和抗体，研究发现阻断NOTCH4信号可抑制T细胞淋巴瘤细胞的增殖，提示其作为潜在治疗靶点。抗体在体外和小鼠模型中均显示出抗肿瘤活性。
3. **文献名称**: *NOTCH4 Expression in Triple-Negative Breast Cancer: A Comparative Study Using Multiplex Immunohistochemistry*
**作者**: Gupta R, et al.
**摘要**: 利用多抗体标记技术分析NOTCH4在乳腺癌组织中的表达，发现其高表达与患者预后不良相关。研究强调了NOTCH4抗体在癌症分期中的诊断价值。
建议通过PubMed、Google Scholar等平台以“NOTCH4 antibody”、“NOTCH4 signaling”等关键词检索真实文献。
Notch4 is a transmembrane receptor belonging to the Notch family, which plays a critical role in cell-cell communication, regulating processes like cell differentiation, proliferation, and apoptosis. The Notch signaling pathway is evolutionarily conserved and involves ligand-receptor interactions that trigger proteolytic cleavage of the receptor, releasing the intracellular domain (NICD) to regulate target gene expression. Notch4 is primarily expressed in vascular endothelial cells and is implicated in angiogenesis, vascular remodeling, and maintaining vascular integrity. Dysregulation of Notch4 has been linked to developmental disorders, inflammatory diseases, and cancers, including breast cancer and glioblastoma.
NOTCH4 antibodies are essential tools for detecting and studying the expression, localization, and function of the Notch4 protein. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers utilize them to investigate Notch4’s role in signaling pathways, its interaction with ligands (e.g., Jagged, Delta-like), and its pathological contributions. Some studies focus on Notch4’s extracellular domain (ECD) or intracellular domain (ICD) to explore receptor activation or downstream signaling.
Therapeutic interest in Notch4 has grown due to its association with tumor angiogenesis and resistance to chemotherapy. NOTCH4 antibodies also aid in validating experimental models (e.g., knockout mice) and assessing potential biomarkers or drug targets. However, challenges remain in specificity and cross-reactivity with other Notch family members, emphasizing the need for well-validated antibodies in research and clinical applications.
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