| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PRKAR1 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRKAR1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRKAR1B抗体的3篇参考文献示例(文献信息为虚构，仅供参考)：
1. **文献名称**：*"Characterization of PRKAR1B Antibody Specificity in cAMP Signaling Pathways"*
**作者**：Smith J, et al.
**摘要**：本研究验证了一种新型PRKAR1B抗体的特异性，通过Western blot和免疫荧光技术证实其在人结肠癌细胞系中特异性识别PRKAR1B蛋白，并揭示其与PKA催化亚基的相互作用调控cAMP信号传导。
2. **文献名称**：*"PRKAR1B Expression in Neuronal Development: Insights from Knockout Mouse Models"*
**作者**：Lee H, et al.
**摘要**：利用PRKAR1B特异性抗体进行免疫组化分析，发现PRKAR1B在小鼠大脑皮层发育中高表达，并通过基因敲除实验证明其缺失导致神经元迁移异常，提示其在神经发育中的关键作用。
3. **文献名称**：*"A Novel PRKAR1B Antibody for Detecting Protein Localization in Cancer Tissues"*
**作者**：Zhang Y, et al.
**摘要**：开发了一种高灵敏度的PRKAR1B多克隆抗体，应用于乳腺癌组织芯片分析，结果显示PRKAR1B在肿瘤间质中的异常表达与患者预后显著相关，为癌症生物标志物研究提供工具。
(注：以上文献为示例，实际研究中请通过PubMed或Google Scholar检索真实文献。)
The PRKAR1B antibody is a research tool designed to detect and analyze the protein kinase cAMP-dependent type I regulatory subunit beta (PRKAR1B), a component of the cAMP-dependent protein kinase (PKA) complex. PKA, a central mediator of cyclic AMP (cAMP) signaling, regulates diverse cellular processes, including metabolism, gene expression, and cell cycle progression. It consists of two regulatory subunits (R) and two catalytic subunits (C). PRKAR1B is one of four regulatory subunit isoforms (R1α, R1β, R2α, R2β) that determine PKA localization, activation, and substrate specificity.
PRKAR1B shares structural homology with PRKAR1A but exhibits distinct tissue expression patterns, with higher levels observed in brain, testis, and certain endocrine tissues. Its specific roles remain less characterized compared to PRKAR1A, though studies suggest involvement in neuronal functions, hormone secretion, and cancer progression. For example, PRKAR1B overexpression has been linked to tumor growth in some contexts.
Antibodies targeting PRKAR1B enable researchers to investigate its expression, subcellular localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Specificity validation is critical, as cross-reactivity with PRKAR1A can occur due to sequence similarity. These antibodies contribute to elucidating PRKAR1B's physiological and pathological roles, particularly in cAMP signaling dysregulation associated with metabolic disorders, neurological conditions, and cancers.
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