| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/3000-1/10000 | Human,Mouse,Rat |
| Aliases | SSeCKS; AKAP250 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AKAP12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AKAP12抗体的3篇参考文献示例(注：内容为模拟概括，实际文献需根据具体检索结果调整)：
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1. **文献名称**：*AKAP12 suppresses tumorigenesis in hepatocellular carcinoma through Wnt/β-catenin signaling inhibition*
**作者**：Li, Y. et al.
**摘要**：研究利用AKAP12抗体进行免疫组化分析，发现AKAP12在肝癌组织中表达下调，并通过抑制Wnt/β-catenin通路抑制肿瘤生长和转移，提示其作为抑癌基因的潜力。
2. **文献名称**：*AKAP12 regulates endothelial cell migration and angiogenesis via interaction with VEGFR2*
**作者**：Choi, M.C. et al.
**摘要**：通过Western blot和免疫荧光(使用AKAP12抗体)，证明AKAP12与VEGFR2结合调控内皮细胞迁移，靶向AKAP12或可抑制病理性血管生成。
3. **文献名称**：*DNA methylation-mediated silencing of AKAP12 promotes colorectal cancer metastasis*
**作者**：Wang, H. et al.
**摘要**：采用AKAP12抗体检测结直肠癌样本，发现启动子甲基化导致AKAP12表达缺失，进而通过EGFR信号通路加速肿瘤侵袭，提示其作为转移标志物的价值。
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建议通过PubMed或Google Scholar以“AKAP12 antibody”、“AKAP12 cancer”等关键词检索最新文献获取详细信息。
The AKAP12 antibody is a crucial tool for studying the A-kinase anchor protein 12 (AKAP12), a scaffold protein involved in organizing signaling complexes within cells. AKAP12. also known as gravin or SSeCKS, regulates key signaling pathways by tethering protein kinases (e.g., PKA, PKC) and phosphatases to specific subcellular locations. It plays roles in cell cycle progression, cytoskeletal organization, and cell adhesion, with emerging links to cancer suppression, metastasis inhibition, and vascular homeostasis.
Researchers use AKAP12 antibodies to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) in tissues or cultured cells. These antibodies are vital in cancer studies, as AKAP12 downregulation is associated with tumor progression in cancers like breast, prostate, and gastric cancer. Its tumor-suppressive functions involve inhibiting angiogenesis, epithelial-mesenchymal transition (EMT), and oncogenic signaling (e.g., Wnt/β-catenin).
Validated in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, AKAP12 antibodies help elucidate its dynamic roles in cellular stress responses and drug resistance. Recent studies also explore AKAP12's involvement in neurological disorders and fibrosis. Specificity validation (e.g., knockout controls) is essential due to isoform diversity and tissue-specific expression patterns. This antibody remains a key reagent for dissecting AKAP12's multifaceted roles in physiology and disease. (299 words)
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