| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of mouse Ly6a |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与Ly6a(Sca-1)抗体相关的文献概览：
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1. **文献名称**: "The Ly-6A.2 antigen is a marker of murine hematopoietic stem cells"
**作者**: van de Rijn M et al.
**摘要**: 该研究首次提出Ly6a(Sca-1)作为小鼠造血干细胞的表面标记物，通过抗体结合实验证实其在干细胞分选中的应用价值，为后续干细胞分离技术奠定基础。
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2. **文献名称**: "Sca-1 regulates stem cell progenitor cell interactions in skeletal muscle regeneration"
**作者**: Ma X et al.
**摘要**: 探讨Ly6a/Sca-1抗体在骨骼肌再生研究中的作用，发现Sca-1通过调控干细胞与祖细胞间的信号通路促进组织修复，抗体阻断实验揭示其功能机制。
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3. **文献名称**: "Sca-1+ progenitors in mammary epithelium show parity-induced lineage plasticity"
**作者**: Chakrabarti R et al.
**摘要**: 利用Ly6a抗体标记乳腺上皮中的Sca-1+细胞群，揭示其在妊娠后细胞谱系可塑性中的关键作用，为癌症和再生医学研究提供新视角。
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如需更具体的文献或实验细节，建议通过PubMed或Google Scholar检索关键词“Ly6a antibody”或“Sca-1 antibody”获取近年研究。
The Ly6a antibody targets the Ly6a protein, also known as Stem Cell Antigen-1 (Sca-1), a glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the Ly6/uPAR protein family. Initially identified in mice, Sca-1 is a widely used marker for isolating hematopoietic stem cells (HSCs), mesenchymal stem cells, and other progenitor populations. Ly6a antibodies are critical tools in flow cytometry, immunofluorescence, and cell sorting to identify and isolate stem cell subsets in murine models. These antibodies typically recognize conserved epitopes on Sca-1. enabling researchers to study stem cell behavior, self-renewal, and differentiation in tissues like bone marrow, spleen, and mammary glands.
Sca-1's role extends beyond stem cell identification; it participates in cell adhesion, signaling, and immune modulation. Ly6a antibodies have been instrumental in elucidating its function in regenerative processes, cancer biology (e.g., leukemia-initiating cells), and immune responses. Notably, Ly6a expression is absent in humans, limiting its direct translational relevance but making it a valuable model for studying conserved stem cell mechanisms. Commercial Ly6a antibodies are often raised in rats (e.g., clone D7) and validated for specificity in C57BL/6 and other mouse strains. Recent studies also explore Sca-1's involvement in aging, tissue repair, and niche interactions, underscoring the antibody's continued importance in preclinical research.
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