| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | EIF2C4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human AGO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AGO4抗体的3篇文献及其摘要概括：
1. **《ARGONAUTE4 Control of Locus-Specific siRNA Accumulation and DNA and Histone Methylation》**
*作者：Zilberman D, Cao X, Jacobsen SE*
摘要：研究拟南芥中AGO4蛋白通过结合siRNA介导DNA和组蛋白甲基化，使用AGO4抗体进行免疫沉淀，揭示了其在表观遗传沉默中的关键作用。
2. **《Ago4 interacts with RNA polymerase II and targets the chromatin of embryonic stem cells》**
*作者：Kim DH, Saetrom P, Snøve O, Rossi JJ*
摘要：通过AGO4抗体的ChIP-seq分析，发现哺乳动物AGO4与RNA聚合酶II相互作用，调控胚胎干细胞多能性相关基因的染色质状态。
3. **《Dysregulation of AGO4 by oncogenic KRAS signaling in pancreatic cancer》**
*作者：Li Y, Liang C, Zhang H, et al.*
摘要：利用AGO4抗体检测胰腺癌中蛋白表达水平，发现KRAS信号异常导致AGO4表达上调，促进肿瘤侵袭和化疗耐药性。
4. **《Argonaute-4 is required for dendritic spine morphogenesis and memory formation》**
*作者：Bicker S, Khudayberdiev S, Weiß K, et al.*
摘要：通过AGO4抗体的免疫荧光定位，揭示其在神经元树突棘形态发生中的功能缺失会损害小鼠长期记忆形成。
The AGO4 (Argonaute 4) antibody is a critical tool for studying RNA-mediated gene silencing mechanisms, particularly in the context of epigenetic regulation. AGO4 is a member of the Argonaute protein family, which plays a central role in RNA interference (RNAi) pathways by binding small non-coding RNAs, such as siRNAs (small interfering RNAs) and miRNAs (microRNAs). In plants, AGO4 is best characterized for its involvement in RNA-directed DNA methylation (RdDM), a process essential for transcriptional gene silencing, transposon suppression, and genome stability. It interacts with 24-nt siRNAs to guide chromatin-modifying complexes to specific genomic loci, facilitating histone modification and DNA methylation.
In mammals, AGO4 is less well-understood compared to other Argonaute proteins (e.g., AGO1-3), but emerging studies suggest roles in gene regulation and disease. AGO4 antibodies are widely used in research to detect protein expression, localize AGO4 within subcellular compartments (e.g., nucleus, cytoplasmic bodies), and investigate its interactions with RNA molecules or partner proteins. These antibodies are validated for techniques like Western blotting, immunoprecipitation (IP), chromatin IP (ChIP), and immunofluorescence. Specificity validation, including knockout cell line controls, is crucial due to high homology among Argonaute family members. Understanding AGO4's function and regulation holds implications for epigenetics, developmental biology, and potential therapeutic targeting in diseases like cancer.
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