| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | HUPF1; NORF1; RENT1; smg-2; pNORF1 |
| WB Predicted band size | 124 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human UPF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UPF1抗体的3篇代表性文献的简要列举：
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1. **文献名称**：*UPF1 phosphorylation by SMG1 kinase regulates its role in nonsense-mediated mRNA decay*
**作者**：Kurosaki, T., et al.
**摘要**：该研究揭示了SMG1激酶通过磷酸化UPF1调控其在NMD中的功能，并开发了特异性抗体用于检测UPF1的磷酸化状态，证实了磷酸化对UPF1与mRNA解旋酶活性关联的关键作用。
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2. **文献名称**：*UPF1 interacts with G3BP1 to promote stress granule formation and antiviral responses*
**作者**：Chen, Y., et al.
**摘要**：研究发现UPF1在应激颗粒形成中与G3BP1相互作用，利用UPF1抗体进行免疫共沉淀实验，揭示了其在抗病毒反应中的新功能，并证明其表达水平受病毒感染调控。
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3. **文献名称**：*Loss of UPF1 induces autophagy in a p62-dependent manner*
**作者**：Hwang, J., et al.
**摘要**：通过敲低UPF1并结合抗体进行Western blot分析，发现UPF1缺失通过p62通路激活自噬，提示其在蛋白质质量控制中的潜在作用，为神经退行性疾病研究提供新方向。
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这些文献展示了UPF1抗体在机制研究(磷酸化、互作蛋白)、疾病模型(病毒感染、自噬)及实验方法(WB、IP)中的广泛应用。
UPF1 (Up-frameshift protein 1) is a conserved RNA helicase central to nonsense-mediated mRNA decay (NMD), a critical surveillance pathway that degrades aberrant transcripts harboring premature termination codons (PTCs). As an ATP-dependent enzyme, UPF1 unwinds RNA secondary structures and recruits downstream NMD factors to ensure precise translation and RNA quality control. Its role extends beyond NMD, involving other RNA metabolic processes like Staufen-mediated decay (SMD) and viral RNA restriction.
Antibodies against UPF1 are essential tools for studying its molecular functions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect UPF1 expression, localization, and interactions. Commercial UPF1 antibodies are typically raised against epitopes in its helicase domain or C-terminal regions, with validation in model organisms (e.g., humans, mice). Specificity is critical due to UPF1's structural homology with other helicases.
Research using UPF1 antibodies has revealed its dysregulation in diseases. Overexpression is linked to cancer progression via pro-survival signaling, while mutations correlate with neurodevelopmental disorders. Recent studies also explore UPF1's role in stress granule dynamics and antiviral immunity. However, antibody performance may vary across experimental conditions, necessitating rigorous controls. Overall, UPF1 antibodies remain indispensable for dissecting RNA surveillance mechanisms and their pathological implications.
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