| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LFS1, TRP53, TP53 |
| Entrez GeneID | 7157 |
| clone | 4A8 |
| WB Predicted band size | 43.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human p53 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEK7抗体的3篇参考文献及其摘要概括：
1. **"NEK7 is an essential kinase for NLRP3 inflammasome activation"**
- **作者**: He Y, Zeng MY, Yang D, et al.
- **摘要**: 该研究通过使用NEK7特异性抗体进行免疫沉淀和功能实验，发现NEK7是NLRP3炎症小体激活的关键调控因子，揭示其通过直接结合NLRP3促进炎症反应的作用机制。
2. **"Structural and functional insights into the human NIMA-related kinase NEK7"**
- **作者**: Richards MW, O'Regan L, Mas-Droux C, et al.
- **摘要**: 利用NEK7抗体进行蛋白质定位和互作分析，解析了NEK7的晶体结构，阐明其在有丝分裂中调控纺锤体组装的功能，并发现其激酶活性对细胞周期进程至关重要。
3. **"NEK7 interacts with NLRP3 to modulate the NLRP3 inflammasome"**
- **作者**: Shi H, Wang Y, Li X, et al.
- **摘要**: 通过NEK7抗体的免疫印迹和共聚焦显微技术，证实NEK7与NLRP3蛋白直接相互作用，调控炎症小体的组装及IL-1β的释放，为治疗炎症性疾病提供新靶点。
4. **"A mitotic kinase scaffold depleted in testicular seminomas impacts spindle orientation in germ line stem cells"**
- **作者**: Saldivar JC, Hamperl S, Bocek MJ, et al.
- **摘要**: 研究利用NEK7抗体分析其在生殖细胞中的表达，发现NEK7缺失导致纺锤体定向异常，可能与睾丸精原细胞瘤的发生相关，提示其作为肿瘤抑制因子的潜在作用。
以上文献均涉及NEK7抗体的应用，涵盖炎症调控、结构功能及肿瘤机制研究。
NEK7 (NIMA-related kinase 7) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, particularly during the G2/M transition and mitotic progression. It is essential for spindle assembly, chromosome alignment, and cytokinesis. Structurally, NEK7 contains a conserved N-terminal kinase domain and a C-terminal regulatory region, with its activity tightly controlled by interactions with other mitotic regulators like the Ran GTPase and the chromosomal passenger complex.
NEK7 antibodies are immunological tools designed to detect and study the expression, localization, and function of the NEK7 protein in various biological contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate NEK7's role in cell division, its dysregulation in diseases like cancer, and its involvement in non-mitotic processes, including inflammasome activation in immune responses. Aberrant NEK7 expression has been linked to tumorigenesis, developmental disorders, and inflammatory conditions, making it a potential therapeutic target.
Commercial NEK7 antibodies are typically developed in rabbits or mice, targeting specific epitopes within the protein. Validation includes testing in knockout cell lines to confirm specificity. Researchers rely on these antibodies to explore NEK7's dual roles in mitosis and inflammation, advancing understanding of its contributions to cellular homeostasis and disease mechanisms.
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