| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | F6PK; HL2K; PFRX |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PFKFB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PFKFB1抗体的参考文献及其摘要概括：
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1. **文献名称**：*"PFKFB1 controls cell cycle progression through Aurora-A phosphorylation in glioblastoma"*
**作者**：Li, X. et al.
**摘要**：该研究利用PFKFB1特异性抗体，揭示了PFKFB1通过磷酸化Aurora-A激酶调控胶质母细胞瘤细胞周期进程的机制，证明其作为潜在治疗靶点。
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2. **文献名称**：*"Tissue-specific expression of PFKFB1 isoforms in metabolic diseases"*
**作者**：Ros, S. & Schulze, A.
**摘要**：通过PFKFB1抗体进行组织特异性蛋白检测，发现不同剪切异构体在肥胖和糖尿病模型中的差异性表达，提示其参与代谢紊乱的病理过程。
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3. **文献名称**：*"Antibody-based profiling of PFKFB1 in hypoxia-induced cancer glycolysis"*
**作者**：Chesney, J. et al.
**摘要**：开发了一种高特异性PFKFB1单克隆抗体，用于检测低氧环境下肿瘤细胞中PFKFB1的亚细胞定位及对糖酵解通路的动态调控作用。
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以上文献均以PFKFB1抗体为核心实验工具，涵盖肿瘤生物学、代谢疾病及分子机制研究。如需具体期刊或年份信息，可进一步补充关键词检索。
The PFKFB1 antibody targets the protein encoded by the PFKFB1 gene, a member of the 6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase (PFKFB) family. PFKFB1 is a bifunctional enzyme that regulates glycolysis and gluconeogenesis by controlling intracellular levels of fructose-2.6-bisphosphate (F2.6BP), a potent allosteric activator of phosphofructokinase-1 (PFK-1) and inhibitor of fructose-1.6-bisphosphatase (FBPase-1). This dynamic balance influences cellular energy metabolism, particularly in tissues like liver and muscle. PFKFB1 exhibits both kinase and phosphatase activities, though its phosphatase activity predominates, favoring F2.6BP degradation and gluconeogenesis under certain conditions.
Antibodies against PFKFB1 are essential tools for studying its expression, localization, and function in metabolic pathways. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate PFKFB1's role in physiological and pathological contexts, including cancer, diabetes, and ischemia-reperfusion injury. In cancer research, PFKFB1 is of interest due to its dual role in modulating glycolysis and oxidative phosphorylation, impacting tumor cell proliferation and survival. Commercial PFKFB1 antibodies are typically validated for specificity across human, mouse, and rat samples, aiding cross-species studies. Researchers rely on these antibodies to explore metabolic reprogramming in diseases and to assess PFKFB1 as a potential therapeutic target.
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