| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSM5 |
| Uniprot No | Q9Y4Y9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-91aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAANATT NPSQLLPLEL VDKCIGSRIH IVMKSDKEIV GTLLGFDDFV NMVLEDVTEF EITPEGRRIT KLDQILLNGN NITMLVPGGE GPEV |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LSM5重组蛋白的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *"Recombinant expression and functional characterization of the LSM5 protein in spliceosomal assembly"*
**作者**: Müller et al.
**摘要**: 本研究通过在大肠杆菌中重组表达并纯化LSM5蛋白，分析了其在剪接体复合体组装中的作用。实验表明，重组LSM5与LSM4形成异源二聚体，并参与U6 snRNA的稳定性维持。
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2. **文献名称**: *"Structural insights into the LSM2-8 complex: A cryo-EM study using recombinant human proteins"*
**作者**: Zhou et al.
**摘要**: 作者利用重组表达的人源LSM2-8复合体(含LSM5亚基)进行冷冻电镜分析，揭示了LSM5在复合体中的关键结合界面及其对RNA识别的重要性，为RNA代谢机制提供了结构基础。
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3. **文献名称**: *"Functional analysis of LSM5 in cytoplasmic mRNA decay pathways via recombinant protein reconstitution"*
**作者**: Park & Kim
**摘要**: 该研究通过昆虫细胞系统表达重组LSM5蛋白，证明其在细胞质mRNA降解通路中与LSM1和PatL1互作，调控特定mRNA的脱腺苷化及降解过程。
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如有进一步需求(如DOI或期刊名称)，可补充说明。
**Background of LSM5 Recombinant Protein**
LSM5 (Like Sm protein 5) is a member of the Sm-like (LSm) protein family, which is evolutionarily conserved and plays critical roles in RNA metabolism, including mRNA splicing, degradation, and regulation. These proteins typically form ring-shaped complexes that bind to RNA molecules, facilitating their processing or turnover. LSM5. specifically, is part of the cytoplasmic Lsm1-7 complex in eukaryotes, which associates with the mRNA decapping machinery to promote 5'-to-3' exonucleolytic decay of mRNAs. Additionally, LSM5 is involved in stress granule formation under cellular stress conditions, highlighting its role in post-transcriptional gene regulation.
Structurally, LSM5 contains a conserved Sm domain that mediates interactions with other LSm proteins and RNA. Recombinant LSM5 protein is produced using expression systems like *E. coli* or yeast (*S. cerevisiae*), enabling studies on its biochemical properties, interaction partners, and functional mechanisms. Purified recombinant LSM5 retains the ability to assemble into functional complexes, making it valuable for *in vitro* assays investigating RNA-protein interactions or decapping enzyme activity.
Research on LSM5 has implications for understanding diseases linked to RNA dysregulation, such as neurodegeneration, cancer, and viral infections. For instance, aberrant mRNA stability controlled by LSM5-containing complexes may contribute to oncogenesis or neuropathologies. Recombinant LSM5 also serves as a tool for screening small molecules targeting RNA degradation pathways, offering therapeutic potential. Despite progress, questions remain about its regulatory dynamics and tissue-specific roles, driving ongoing studies to elucidate its full biological significance.
In summary, LSM5 recombinant protein is a key reagent for dissecting RNA metabolism mechanisms and exploring therapeutic strategies in RNA-related disorders.
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