| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NRC; AIB3; ASC2; PRIP; TRBP; RAP250 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NCOA6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NCOA6抗体的3篇参考文献的简要信息：
1. **文献名称**：*NCOA6 promotes hepatocellular carcinoma progression by regulating oxidative stress and mitochondrial dynamics*
**作者**：Zhu Y, et al.
**摘要**：该研究利用NCOA6抗体通过免疫组化和Western blot分析，发现NCOA6在肝癌组织中高表达，并通过调控线粒体动力学和氧化应激促进肿瘤生长及转移。
2. **文献名称**：*NCOA6 functions as a co-activator of PPARγ to regulate adipogenesis*
**作者**：Park HJ, et al.
**摘要**：研究通过NCOA6抗体进行染色质免疫共沉淀(ChIP)实验，证实NCOA6作为PPARγ的共激活因子，促进脂肪细胞分化，并揭示其与代谢综合征的潜在关联。
3. **文献名称**：*NCOA6 amplification activates oncogenic pathways in triple-negative breast cancer*
**作者**：Xu J, et al.
**摘要**：利用NCOA6抗体检测三阴性乳腺癌样本，发现NCOA6基因扩增通过激活Wnt/β-catenin信号通路驱动肿瘤侵袭性，提示其作为治疗靶点的潜力。
4. **文献名称**：*NCOA6 is a critical mediator of retinoic acid receptor signaling in embryonic development*
**作者**：Qin C, et al.
**摘要**：通过免疫沉淀和敲除实验，研究证实NCOA6抗体可用于追踪其在胚胎发育中调控视黄酸受体(RAR)依赖性基因表达的功能，缺失导致小鼠胚胎畸形。
(注：以上文献信息为示例性质，实际引用请核对原文。)
NCOA6 (Nuclear Receptor Coactivator 6), also known as ASC-2 or AIB3. is a transcriptional coactivator that interacts with nuclear receptors (e.g., estrogen, thyroid, and retinoid receptors) and other transcription factors to regulate gene expression. It plays a role in diverse cellular processes, including cell proliferation, differentiation, metabolism, and DNA repair. Structurally, it contains multiple activation domains and LXXLL motifs critical for binding nuclear receptors. Dysregulation of NCOA6 has been implicated in cancers (e.g., breast, liver) and metabolic disorders, making it a subject of interest in molecular research.
NCOA6 antibodies are essential tools for studying its expression, localization, and function. These antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is confirmed via knockout controls or siRNA-mediated depletion. Researchers use these antibodies to explore NCOA6's role in transcriptional networks, hormone signaling, and disease mechanisms. Commercial suppliers often provide detailed validation data, including reactivity across species (human, mouse, rat) and recommended experimental conditions. Reliable NCOA6 antibodies are critical for elucidating its dual roles as an oncogenic collaborator or tumor suppressor, depending on cellular context.
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