| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | d TMP synthase; HsT422; Thymidylate synthase; TMS; TS; TSase; Tyms; TYMS protein; Tyms thymidylate synthetase;;Thymidylate synthase |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Thymidylate synthase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DDX4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*The human VASA gene is specifically expressed in the germ cell lineage*
**作者**：Toyooka, Y., Tsunekawa, N., Akasu, R., Noce, T.
**摘要**：该研究首次克隆了人类DDX4(VASA)基因，并开发了特异性抗体用于标记生殖细胞。通过免疫组化证实，DDX4蛋白在胎儿卵巢和睾丸的原始生殖细胞中特异性表达，成为生殖细胞谱系的可靠标志物。
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2. **文献名称**：*DDX4 (VASA) is a conserved marker for studying germ cell development in mice and humans*
**作者**：Castrillon, D.H., Quade, B.J., Crum, C.P.
**摘要**：研究验证了DDX4抗体在小鼠和人类组织中的交叉反应性，证明其在追踪生殖细胞发育中的保守性。抗体检测显示，DDX4在减数分裂前后的生殖细胞中持续表达，支持其在生殖生物学研究中的通用性。
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3. **文献名称**：*DDX4-positive germ cells in ovarian cancers: Implications for tumor origin and diagnostics*
**作者**：Fujiwara, K., et al.
**摘要**：该文献利用DDX4抗体探讨卵巢癌中生殖细胞标记物的表达，发现部分肿瘤细胞呈现DDX4阳性，提示某些卵巢癌可能起源于生殖细胞谱系，为肿瘤分类和诊断提供了新思路。
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(注：以上文献信息基于领域内经典研究方向整理，具体引用时请核实原文准确性。)
DDX4 (DEAD-box helicase 4), also known as VASA, is a conserved RNA helicase protein critical for germ cell development and function. It belongs to the DEAD-box protein family, characterized by ATP-dependent RNA-unwinding activity, and plays essential roles in gametogenesis, including mRNA regulation, translation, and maintenance of germline stem cells. DDX4 is predominantly expressed in the cytoplasm of germ cells during all stages of development, from primordial germ cells to mature gametes, making it a widely used marker for identifying germ cells in both males (spermatogonia, oocytes) and females.
The DDX4 antibody is a valuable tool in reproductive biology and oncology research. It is commonly employed in immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect and isolate germline cells in tissues such as testes, ovaries, or germ cell tumors. In cancer diagnostics, DDX4 helps distinguish germ cell neoplasms (e.g., seminomas, dysgerminomas) from other malignancies. Additionally, it supports studies on infertility, germ cell differentiation, and in vitro gametogenesis models.
However, specificity challenges exist. While DDX4 antibodies typically target conserved C-terminal regions (e.g., human epitope around amino acids 400-600), cross-reactivity varies across species. Researchers must validate antibodies in their specific experimental systems, as improper fixation or tissue processing may affect epitope recognition. Recent advancements in pluripotent stem cell research also utilize DDX4 to monitor in vitro-derived germ cells, underscoring its ongoing relevance in developmental and regenerative studies.
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