首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白B(APOB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOB含量。载脂蛋白B（APOB）是一种在肝脏合成的蛋白质，主要存在于乳糜微粒、低密度脂蛋白和极低密度脂蛋白中，负责脂类的转运。研究显示，APOB水平与动脉粥样硬化和心血管疾病风险密切相关，其研究机制涉及脂质代谢、炎症反应和血管病变等多个方面。试剂盒检测范围为0.156 μg/mL-10 μg/mL，灵敏度为0.039 μg/mL。支持科研人员高效开展小鼠模型中的脂代谢机制研究、动脉粥样硬化相关实验或药物干预效果评估，为探索APOB在脂质代谢调控中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ApobApolipoprotein B-100 ELISA kit; Apo B-100) [Cleaved into: Apolipoprotein B-48 ELISA kit; Apo B-48)] ELISA kit
缩写：APOB
Uniprot No.：E9Q414
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 102 | ||||
| Range % | 97-108 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 97-106 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:800 | Average % | 86 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of mouse APOB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 105 | 102-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 100.0910.0920.092 50.1360.1320.134 2.50.2360.2290.233 1.250.3600.3680.364 0.6250.5390.5240.532 0.3120.8770.9270.902 0.1561.3341.2861.310 02.5622.7472.655 | ||||||
本试剂盒所含材料：
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A 96-well Assay plate --The 96-well plate has been pre-coated with ApoB.
Two pieces of Standard (Freeze-dried)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated ApoB antibody (100 x concentrate) (1 x 60μl) --Act as the detection antibody.
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated ApoB antibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
本试剂盒不含材料：
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A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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