| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GYPC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **HACE1抗体** 的3篇示例参考文献(内容为模拟虚构，供参考格式)：
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1. **文献名称**: *HACE1 regulates oxidative stress resistance by ubiquitinating mitochondrial SOD2 in cancer cells*
**作者**: Smith A, et al.
**摘要**: 本研究揭示了HACE1通过泛素化线粒体超氧化物歧化酶SOD2调控肿瘤细胞的氧化应激应答。使用HACE1特异性抗体进行免疫沉淀实验，发现HACE1缺失导致SOD2稳定性增加，促进癌细胞耐药性。
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2. **文献名称**: *HACE1 deficiency promotes neurodegeneration through impaired autophagy-lysosomal pathways*
**作者**: Chen L, et al.
**摘要**: 通过构建HACE1敲除小鼠模型并结合抗体免疫组化分析，发现HACE1缺失导致神经元自噬功能障碍，加速α-突触核蛋白聚集，与帕金森病病理进程密切相关。
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3. **文献名称**: *Antibody-based profiling of HACE1 expression in colorectal cancer progression*
**作者**: Gupta R, et al.
**摘要**: 开发了一种高特异性HACE1单克隆抗体，用于结直肠癌组织芯片分析。结果显示HACE1蛋白表达与肿瘤分期呈负相关，提示其作为抑癌基因的潜在临床标志物作用。
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**备注**：以上文献为示例，实际研究中建议通过PubMed、Google Scholar等平台检索关键词“HACE1 antibody”或“HACE1 function”获取真实文献。
The HACE1 (HECT domain and ankyrin repeat containing E3 ubiquitin-protein ligase 1) antibody is a tool used to detect and study the HACE1 protein, a ubiquitin ligase involved in cellular regulation and disease. HACE1 functions as an E3 ubiquitin ligase, tagging specific substrates with ubiquitin for proteasomal degradation. It plays critical roles in tumor suppression, oxidative stress response, and maintaining cellular homeostasis. Structurally, it contains an N-terminal ankyrin repeat domain for protein interactions and a C-terminal HECT domain responsible for its enzymatic activity.
Research links HACE1 to cancer biology, as its gene is frequently downregulated or deleted in various cancers, including Wilms' tumor, breast cancer, and lung adenocarcinoma. Loss of HACE1 correlates with increased tumor growth, metastasis, and poor prognosis, attributed to dysregulation of its substrates like Rac1. a GTPase involved in cell motility and invasion. HACE1-mediated ubiquitination of Rac1 limits its activity, thereby suppressing tumor-promoting signaling pathways.
HACE1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and interactions in cellular and tissue samples. Studies also explore its role in neurodegenerative diseases and metabolic disorders, highlighting its broad regulatory influence. Knockout mouse models further demonstrate HACE1's importance in preventing spontaneous tumors and oxidative damage. Overall, HACE1 antibodies are vital for unraveling the protein's multifaceted roles in health and disease.
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