| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HMGCS |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HMGCS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGCS1抗体的3篇示例参考文献(内容为模拟示例，仅供参考)：
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1. **文献名称**: *HMGCS1-mediated ketogenesis promotes hepatocellular carcinoma progression*
**作者**: Li et al., 2020
**摘要**: 研究通过Western blot和免疫组化(使用HMGCS1抗体)发现，HMGCS1在肝癌组织中高表达，其介导的酮体生成通过调控能量代谢促进肿瘤生长，提示其作为潜在治疗靶点。
2. **文献名称**: *HMGCS1 regulates lipid metabolism in glioblastoma via the AMPK pathway*
**作者**: Smith et al., 2018
**摘要**: 利用HMGCS1特异性抗体进行蛋白质定位分析，发现其在胶质母细胞瘤细胞线粒体中富集，并通过AMPK通路调控脂质代谢，影响肿瘤细胞存活。
3. **文献名称**: *Antibody-based profiling of HMGCS1 in ovarian cancer stem cells*
**作者**: Garcia-Ruiz et al., 2021
**摘要**: 研究通过流式细胞术和免疫荧光(基于HMGCS1单克隆抗体)证实HMGCS1在卵巢癌干细胞中高表达，与化疗耐药性和胆固醇合成异常相关。
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(注：以上为模拟生成的参考文献，实际文献需通过PubMed、Google Scholar等平台检索确认。)
The HMGCS1 (3-hydroxy-3-methylglutaryl-CoA synthase 1) antibody is a crucial tool for studying the enzyme HMGCS1. a rate-limiting mitochondrial protein involved in ketogenesis and cholesterol biosynthesis. HMGCS1 catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA, a key intermediate in these metabolic pathways. Dysregulation of HMGCS1 has been linked to metabolic disorders, cancers (e.g., hepatocellular carcinoma), and neurodegenerative diseases, making it a research focus in metabolism and disease mechanisms.
HMGCS1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and tissue distribution. Their specificity is critical for distinguishing HMGCS1 from its cytosolic isoform HMGCS2. which functions in ketone body production during fasting. Validated antibodies help elucidate HMGCS1's role in cellular energy homeostasis, lipid metabolism, and disease progression.
Commercial HMGCS1 antibodies are typically raised against peptide sequences unique to the human or murine protein, with applications in both basic research and clinical studies. Their utility extends to exploring therapeutic strategies targeting cholesterol synthesis pathways, particularly in cancer and cardiovascular diseases. Proper validation using knockout controls or siRNA knockdown is essential to ensure antibody reliability in experimental models.
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