| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GASC1; JHDM3C; JMJD2C; TDRD14C; bA146B14.1 |
| WB Predicted band size | 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KDM4C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KDM4C抗体的3篇参考文献示例(注：以下内容为模拟虚构，仅供参考)：
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1. **文献名称**：*KDM4C-mediated epigenetic silencing promotes prostate cancer progression by activating Wnt signaling*
**作者**：J. Luo et al.
**摘要**：本研究利用KDM4C特异性抗体进行免疫组化(IHC)和染色质免疫沉淀(ChIP)，揭示了KDM4C通过去甲基化H3K9me3激活Wnt通路基因，促进前列腺癌细胞侵袭。抗体验证显示KDM4C在转移性肿瘤中高表达，提示其作为预后标志物的潜力。
2. **文献名称**：*Development of a high-affinity monoclonal antibody for KDM4C and its application in breast cancer models*
**作者**：S. Yamaguchi, T. Nakamura
**摘要**：研究者开发了一种新型KDM4C单克隆抗体，通过Western blot和免疫荧光验证其特异性。该抗体成功用于检测乳腺癌细胞系中KDM4C的过表达，并证实其与ERα信号通路的相互作用，为靶向治疗提供了工具支持。
3. **文献名称**：*KDM4C regulates hypoxia-induced gene expression via histone demethylation*
**作者**：C. B. Madsen, H. Johansen
**摘要**：通过ChIP-seq结合KDM4C抗体，本文发现KDM4C在缺氧条件下靶向HIF-1α调控的基因启动子区域，去除H3K9me3标记以增强转录。抗体特异性实验证实了KDM4C在胶质母细胞瘤中与患者生存率负相关。
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**说明**：上述文献为示例性质，实际引用需以真实发表的论文为准。如需查找真实文献，建议在PubMed或Web of Science中搜索关键词(如“KDM4C antibody”、“JMJD2C inhibitor”)，并筛选涉及抗体开发、验证或应用的实验研究。
The KDM4C antibody is a crucial tool in studying the lysine demethylase 4C (KDM4C), also known as JMJD2C or GASC1. a member of the KDM4/JMJD2 histone demethylase family. KDM4C specifically catalyzes the removal of methyl groups from histone H3 lysine 9 trimethylation (H3K9me3) and H3 lysine 36 trimethylation (H3K36me3), modulating chromatin structure and gene expression. This enzyme plays pivotal roles in epigenetic regulation, influencing processes like transcriptional activation, heterochromatin maintenance, DNA repair, and cell cycle progression. Dysregulation of KDM4C is linked to cancers, developmental disorders, and metabolic diseases due to its oncogenic potential in promoting tumor growth, metastasis, and therapy resistance.
KDM4C antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). They aid in exploring KDM4C's role in disease mechanisms, drug discovery, and biomarker studies. Validated antibodies are essential for specificity, as the KDM4 family shares structural homology. Researchers often verify antibody performance using knockout cell lines or siRNA-mediated KDM4C depletion. Recent studies also focus on developing KDM4C inhibitors, making these antibodies vital for preclinical evaluation of targeted therapies aiming to restore epigenetic balance in malignancies.
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