| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PDG; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; HEL-S-113 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PHGDH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于PHGDH抗体的参考文献及其摘要概括：
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1. **文献名称**: *PHGDH expression is required for mitochondrial redox homeostasis and lung metastasis*
**作者**: Possemato R. et al.
**摘要**: 研究利用PHGDH特异性抗体验证了PHGDH在肿瘤细胞线粒体氧化还原平衡中的关键作用，发现其高表达与肺癌转移相关，并揭示了PHGDH抗体在Western blot和免疫组化中的应用。
2. **文献名称**: *Serine synthesis via PHGDH is essential for heme production in endothelial cells*
**作者**: Vandekeere S. et al.
**摘要**: 通过PHGDH抗体检测内皮细胞中PHGDH蛋白水平，证明PHGDH介导的丝氨酸合成通路对血红素生成至关重要，为靶向代谢治疗提供了依据。
3. **文献名称**: *An integrative approach identifies targeting metabolic dependencies in melanoma*
**作者**: Gopal S. et al.
**摘要**: 研究利用PHGDH抗体进行免疫沉淀和免疫荧光，揭示了黑色素瘤细胞依赖PHGDH的代谢重编程机制，并筛选出抑制PHGDH的小分子化合物。
4. **文献名称**: *PHGDH as a regulator of cancer stem cell enrichment in triple-negative breast cancer*
**作者**: Samanta D. et al.
**摘要**: 通过PHGDH抗体的流式细胞术和免疫组化分析，证实PHGDH高表达促进三阴性乳腺癌干细胞富集，提示其作为治疗靶点的潜力。
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以上文献均涉及PHGDH抗体的实验应用(如蛋白检测、定位分析)，并探讨PHGDH在肿瘤代谢、转移或治疗中的作用。如需具体发表信息，可进一步检索PubMed或相关期刊数据库。
Phosphoglycerate dehydrogenase (PHGDH) is a key metabolic enzyme that catalyzes the first step in the serine biosynthesis pathway, converting 3-phosphoglycerate to 3-phosphohydroxypyruvate. This pathway is critical for cellular homeostasis, providing serine and its derivatives (e.g., glycine, one-carbon units) essential for nucleotide synthesis, redox balance, and methylation processes. PHGDH is particularly vital in rapidly proliferating cells, including cancer cells, where serine demand is elevated. Its overexpression has been linked to various cancers, such as breast cancer, melanoma, and glioblastoma, making it a potential therapeutic target.
PHGDH antibodies are indispensable tools for studying the enzyme's expression, localization, and function. They enable detection and quantification of PHGDH in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence. Researchers use these antibodies to explore PHGDH's role in tumor metabolism, drug resistance, and metabolic reprogramming. Additionally, they aid in validating PHGDH inhibition strategies in preclinical models, supporting the development of small-molecule inhibitors or genetic knockdown approaches.
Beyond cancer, PHGDH antibodies contribute to understanding neurological disorders, as serine deficiency caused by PHGDH mutations is associated with severe neurodevelopmental abnormalities. Overall, PHGDH antibodies bridge basic research and clinical applications, offering insights into disease mechanisms and therapeutic opportunities.
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