| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | HL14 |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human LGALS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PHKG2抗体的参考文献示例(注：以下内容为模拟文献，实际引用时请核实真实来源)：
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1. **"PHKG2 mutations and glycogen storage disease IX: A clinical and molecular study"**
*作者：Maurya S, et al. (2015)*
**摘要**：研究利用PHKG2特异性抗体分析患者肝组织，发现PHKG2基因突变导致蛋白表达缺失，与糖原贮存病IX型的代谢异常相关。
2. **"Dysregulation of PHKG2 in hepatocellular carcinoma: Role in metabolic reprogramming"**
*作者：Zhang Y, et al. (2018)*
**摘要**：通过PHKG2抗体进行免疫组化和Western blot，揭示肝癌组织中PHKG2表达下调，其缺失促进糖原累积并加速肿瘤生长。
3. **"Validation of a PHKG2-specific antibody for skeletal muscle phosphorylation studies"**
*作者：Brown KE, et al. (2020)*
**摘要**：验证了PHKG2抗体的特异性，并应用于骨骼肌样本，证明运动后PHKG2磷酸化水平升高，调控糖原分解活性。
4. **"PHKG2 modulates cardiac hypertrophy via energy metabolism pathways"**
*作者：Lee H, et al. (2019)*
**摘要**：使用PHKG2抗体检测心肌细胞，发现压力超负荷模型中PHKG2表达上调，可能通过改变磷酸化酶活性影响心力衰竭进程。
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**提示**：实际研究中，建议通过PubMed或Google Scholar以关键词“PHKG2 antibody”或“PHKG2 immunohistochemistry”检索最新文献，并优先选择经过抗体验证(如KO验证)的研究。
The PHKG2 (Phosphorylase Kinase Gamma 2) antibody is a tool used to study the gamma-2 regulatory subunit of phosphorylase kinase, an enzyme critical for glycogen metabolism. PHKG2. encoded by the *PHKG2* gene, is part of the phosphorylase kinase complex, which activates glycogen phosphorylase to initiate glycogen breakdown. This complex comprises four subunits (α, β, γ, δ), with the γ-subunit (PHKG2) directly binding ATP to catalyze the phosphorylation and activation of glycogen phosphorylase. PHKG2 is primarily expressed in the liver and testis, suggesting roles beyond glycogenolysis, including potential involvement in sperm maturation or male fertility.
Mutations in *PHKG2* are linked to glycogen storage disease type IX (GSD IX), a rare inherited disorder characterized by hepatomegaly, growth delays, and fasting hypoglycemia due to impaired glycogen breakdown. Research using PHKG2 antibodies focuses on detecting protein expression in tissues, assessing its regulatory mechanisms, and exploring its pathological role in metabolic disorders. These antibodies are typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence.
In cancer biology, altered PHKG2 expression has been observed in hepatocellular carcinoma, though its exact role remains unclear. PHKG2 antibodies help investigate its interaction with other kinases or signaling pathways, contributing to broader studies on metabolic reprogramming in diseases. Specificity is crucial, as PHKG2 shares homology with PHKG1 (the gamma-1 isoform), requiring antibodies that distinguish between these isoforms. Overall, PHKG2 antibodies are vital for dissecting glycogen metabolism regulation and its implications in genetic and acquired diseases.
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